FITC Anti-CD28 antibody [PV-1] (ab24844)
![Flow Cytometry - FITC Anti-CD28 antibody [PV-1] (ab24844)](https://www.abcam.com/ps/products/24/ab24844/Images/ab24844-35658-anti-cd28-antibody-pv-1-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD28 antibody [PV-1] (ab24844)")
Key features and details
- FITC Armenian hamster monoclonal [PV-1] to CD28
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-CD28 antibody [PV-1]
See all CD28 primary antibodies -
Description
FITC Armenian hamster monoclonal [PV-1] to CD28 -
Host species
Armenian hamster -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
PV-1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab24844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 3µg for 106 cells. ab18473 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
Tissue specificity
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12487 Mouse
- SwissProt: P31041 Mouse
- Unigene: 255003 Mouse
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Alternative names
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
see all
Images
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Flow Cytometry - FITC Anti-CD28 antibody [PV-1] (ab24844)
ab24844 at a concentration of 3 µg/106 cells staining human A 20 (B cell lymphoma cells) and EL-4 cells by flow cytometry. A 50:50 mixture of the A20 B-cell lymphoma and the EL-4 T-cell lymphoma was stained with ab24844. Large cells were gated and analyzed by flow cytometry.
References (0)
ab24844 has not yet been referenced specifically in any publications.
