FITC Anti-CD28 antibody [PV-1] (ab24844)
![Flow Cytometry - FITC Anti-CD28 antibody [PV-1] (ab24844)](https://www.abcam.com/ps/products/24/ab24844/Images/ab24844-35658-anti-cd28-antibody-pv-1-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD28 antibody [PV-1] (ab24844)")
Key features and details
- FITC Armenian hamster monoclonal [PV-1] to CD28
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
FITC Anti-CD28 antibody [PV-1]
See all CD28 primary antibodies -
Description
FITC Armenian hamster monoclonal [PV-1] to CD28 -
Host species
Armenian hamster -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Tissue, cells or virus corresponding to Mouse CD28. C57BL/6N mouse T cell lymphoma EL4 cell line
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
PV-1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 3µg for 106 cells.
ab18473 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use 3µg for 106 cells. ab18473 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
Tissue specificity
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 940 Human
- Entrez Gene: 12487 Mouse
- Omim: 186760 Human
- SwissProt: P10747 Human
- SwissProt: P31041 Mouse
- Unigene: 591629 Human
- Unigene: 255003 Mouse
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Alternative names
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
see all
Images
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Flow Cytometry - FITC Anti-CD28 antibody [PV-1] (ab24844)
ab24844 at a concentration of 3 µg/106 cells staining human A 20 (B cell lymphoma cells) and EL-4 cells by flow cytometry. A 50:50 mixture of the A20 B-cell lymphoma and the EL-4 T-cell lymphoma was stained with ab24844. Large cells were gated and analyzed by flow cytometry.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab24844 has not yet been referenced specifically in any publications.