FITC Anti-CD3 antibody [CD3-12] (ab34722)
Key features and details
- FITC Rat monoclonal [CD3-12] to CD3
- Suitable for: Flow Cyt
- Reacts with: Mouse, Horse, Chicken, Dog, Human, Pig, Monkey, Rhesus monkey
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
FITC Anti-CD3 antibody [CD3-12]
See all CD3 primary antibodies -
Description
FITC Rat monoclonal [CD3-12] to CD3 -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab34722 recognises a highly conserved epitope of the CD3 molecule expressed by T lymphocytes. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Horse, Chicken, Dog, Human, Pig, Monkey, Rhesus monkey -
Immunogen
Synthetic peptide:
ERPPVPNPDYEPC
Sequence derived from cytoplasmic epitope of CD3. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
CD3-12 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/5 - 1/10.
ab18404 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use 10ul of the suggested working dilution to label 106 cells in 100ul. Membrane permeabilisation is required for this application. |
| Notes |
|---|
|
Flow Cyt
1/5 - 1/10. ab18404 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use 10ul of the suggested working dilution to label 106 cells in 100ul. Membrane permeabilisation is required for this application. |
Target
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Function
The CD3 complex mediates signal transduction. -
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
Sequence similarities
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Entrez Gene: 12500 Mouse
- Entrez Gene: 12501 Mouse
- Entrez Gene: 12502 Mouse
- Entrez Gene: 12503 Mouse
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Datasheets and documents
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Datasheet download
References (0)
ab34722 has not yet been referenced specifically in any publications.
