FITC Anti-CD3 antibody [CD3-12] (ab34722)
Key features and details
- FITC Rat monoclonal [CD3-12] to CD3
- Reacts with: Mouse, Horse, Chicken, Dog, Human, Pig, Monkey, Rhesus monkey
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
FITC Anti-CD3 antibody [CD3-12]
See all CD3 primary antibodies -
Description
FITC Rat monoclonal [CD3-12] to CD3 -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab34722 recognises a highly conserved epitope of the CD3 molecule expressed by T lymphocytes. -
Species reactivity
Reacts with: Mouse, Horse, Chicken, Dog, Human, Pig, Monkey, Rhesus monkey -
Immunogen
Synthetic peptide:
ERPPVPNPDYEPC
Sequence derived from cytoplasmic epitope of CD3. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
CD3-12 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Application notes
FACS: 1/5 - 1/10. Use 10ul of the suggested working dilution to label 106 cells in 100ul. Membrane permeabilisation is required for this application.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
The CD3 complex mediates signal transduction. -
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
Sequence similarities
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Entrez Gene: 12500 Mouse
- Entrez Gene: 12501 Mouse
- Entrez Gene: 12502 Mouse
- Entrez Gene: 12503 Mouse
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Datasheets and documents
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Datasheet download
References (0)
ab34722 has not yet been referenced specifically in any publications.
