FITC Anti-CD3 antibody [UCHT1] (ab34275)
![Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)](https://www.abcam.com/ps/products/34/ab34275/Images/ab34275-276479-anti-cd3-antibody-ucht1-fitc-flow-cytometry.png "Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)")
Key features and details
- FITC Mouse monoclonal [UCHT1] to CD3
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD3 antibody [UCHT1]
See all CD3 primary antibodies -
Description
FITC Mouse monoclonal [UCHT1] to CD3 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
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Immunogen
Tissue/ cell preparation (Human) -infant thymocytes and lymphocytes from a patient with Sezary Syndrome.
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Positive control
- human peripheral blood lymphocytes
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant -
Clonality
Monoclonal -
Clone number
UCHT1 -
Myeloma
P3-NS1/1-Ag4-1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP Anti-CD3 antibody [UCHT1], prediluted (ab106215)
- PE/Cy5® Anti-CD3 antibody [UCHT1], prediluted (ab157300)
- PerCP/Cy5.5® Anti-CD3 antibody [UCHT1], prediluted (ab157317)
- Biotin Anti-CD3 antibody [UCHT1] (ab191112)
- APC Anti-CD3 antibody [UCHT1] (ab239287)
- redFluor™ 710 Anti-CD3 antibody [UCHT1] (ab241935)
- violetFluor™ 450 Anti-CD3 antibody [UCHT1] (ab241941)
- PE/Cy7® Anti-CD3 antibody [UCHT1] (ab81992)
- APC/Cy7® Anti-CD3 antibody [UCHT1] (ab82013)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (5) |
Use at an assay dependent concentration.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
The CD3 complex mediates signal transduction. -
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
Sequence similarities
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Omim: 186740 Human
- Omim: 186780 Human
- Omim: 186790 Human
- Omim: 186830 Human
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)
Flow Cytometry analysis of Human peripheral blood lymphocytes staining CD3 with ab34275 at 5µL (1.0µg) concentration (solid line), and isotype control Mouse IgG1 (FITC) at 1.0µg (dotted line).
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![Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)](https://www.abcam.com/ps/products/34/ab34275/Images/ab34275-47628-anti-cd3-antibody-ucht1-fitc-flow-cytometry.jpg)
Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)This image is courtesy of an anonymous Abreviewab34275 staining CD3 in Human PBMCs by Flow Cytometry. PBMCs were isolated from buffy coat after Ficoll preparation in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
Gating Strategy: Live Lymphocytes. -
![Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)](https://www.abcam.com/ps/products/34/ab34275/Images/ab34275-276389-anti-cd3-antibody-ucht1-fitc-flow-cytometry.png)
Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)FACS analysis of CD3 expression in human peripheral blood lymphocytes stained with ab34275 (solid line) at 0.25 ug and 0.25 ug isotype control (dashed line).
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab34275 has been referenced in 8 publications.
- Di N et al. Effect of combined propofol-sevoflurane anesthesia on immune function in pediatric patients with acute lymphoblastic leukemia. Oncol Lett 18:35-42 (2019). PubMed: 31289469
- Garofalo M et al. Extracellular vesicles enhance the targeted delivery of immunogenic oncolytic adenovirus and paclitaxel in immunocompetent mice. J Control Release 294:165-175 (2019). PubMed: 30557650
- Wang N et al. A Cisplatin-Loaded Immunochemotherapeutic Nanohybrid Bearing Immune Checkpoint Inhibitors for Enhanced Cervical Cancer Therapy. Angew Chem Int Ed Engl 57:3426-3430 (2018). PubMed: 29405579
- Abu N et al. Subchronic toxicity, immunoregulation and anti-breast tumor effect of Nordamnacantal, an anthraquinone extracted from the stems of Morinda citrifolia L. BMC Complement Altern Med 18:31 (2018). PubMed: 29374471
- Wei Y et al. Assessment of tumor growth factor-ß1 neutralizing antibody in the treatment of allergic rhinitis and asthma. Exp Ther Med 15:649-656 (2018). PubMed: 29399067
- Hu Y et al. Effect of TGF-ß1 on blood CD4+CD25high regulatory T cell proliferation and Foxp3 expression during non-small cell lung cancer blood metastasis. Exp Ther Med 16:1403-1410 (2018). PubMed: 30112067
- Garofalo M et al. Systemic Administration and Targeted Delivery of Immunogenic Oncolytic Adenovirus Encapsulated in Extracellular Vesicles for Cancer Therapies. Viruses 10:N/A (2018). PubMed: 30322158
- Harwood SL et al. ATTACK, a novel bispecific T cell-recruiting antibody with trivalent EGFR binding and monovalent CD3 binding for cancer immunotherapy. Oncoimmunology 7:e1377874 (2017). PubMed: 29296540