FITC Anti-CD3 epsilon antibody [C363.29B] (ab24948)
Key features and details
- FITC Rat monoclonal [C363.29B] to CD3 epsilon
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2c
Overview
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Product name
FITC Anti-CD3 epsilon antibody [C363.29B]
See all CD3 epsilon primary antibodies -
Description
FITC Rat monoclonal [C363.29B] to CD3 epsilon -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
This antibody recognises CD3 epsilon chain. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD3 epsilon. IL-4 producing Th2 cell lines including D10.
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Epitope
This antibody reacts with an epitope located in the epsilon chain of the CD3/TCR complex. -
Positive control
- Flow Cyt: Cells from BALB/c mouse mesenteric lymph nodes. Mouse splenocytes.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
C363.29B -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab24948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt | Use 3µg for 106 cells. In vitro depletion of CD3+ cells and activation of T cells (accessory cells must be provided). |
Target
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12501 Mouse
- SwissProt: P22646 Mouse
- Unigene: 210361 Mouse
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Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
Images
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Flow Cytometry analysis of C57BL/6 mouse splenocytes stained with Anti-CD3 epsilon antibody [C363.29B] (FITC) ab24948 (1 µg/106 cells) and Rat Anti-Mouse CD19-PE.
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Ab24948 (3µg for 106 cells) and anti mouse CD45R/B220 RPE conjugate antibody doubly staining cells from BALB/c mesenteric lymph nodes, gated on small lymphocytes and analyzed using a flow cytometer.
Protocols
References (3)
ab24948 has been referenced in 3 publications.
- Mohamad NE et al. Apoptosis and metastasis inhibitory potential of pineapple vinegar against mouse mammary gland cells in vitro and in vivo. Nutr Metab (Lond) 16:49 (2019). PubMed: 31372176
- Zulkawi N et al. In vitro characterization and in vivo toxicity, antioxidant and immunomodulatory effect of fermented foods; Xeniji™. BMC Complement Altern Med 17:344 (2017). Flow Cyt ; Mouse . PubMed: 28666436
- Muraille E et al. Direct visualization of peptide/MHC complexes at the surface and in the intracellular compartments of cells infected in vivo by Leishmania major. PLoS Pathog 6:e1001154 (2010). PubMed: 20976202