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Immunology Adaptive Immunity T Cells CD
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FITC Anti-CD3 epsilon antibody [C363.29B] (ab24948)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (3)

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Flow Cytometry - FITC Anti-CD3 epsilon antibody [C363.29B] (ab24948)
  • Flow Cytometry - FITC Anti-CD3 epsilon antibody [C363.29B] (ab24948)

Key features and details

  • FITC Rat monoclonal [C363.29B] to CD3 epsilon
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG2c

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Overview

  • Product name

    FITC Anti-CD3 epsilon antibody [C363.29B]
    See all CD3 epsilon primary antibodies
  • Description

    FITC Rat monoclonal [C363.29B] to CD3 epsilon
  • Host species

    Rat
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse CD3 epsilon. IL-4 producing Th2 cell lines including D10.

  • Epitope

    This antibody reacts with an epitope located in the epsilon chain of the CD3/TCR complex.
  • Positive control

    • Flow Cyt: Cells from BALB/c mouse mesenteric lymph nodes. Mouse splenocytes.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.4
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    C363.29B
  • Isotype

    IgG2c
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Murine Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • PE Anti-CD3 epsilon antibody [C363.29B] (ab25380)
    • PE/Cy5® Anti-CD3 epsilon antibody [C363.29B] (ab25531)
  • Isotype control

    • FITC Rat IgG2c, kappa [RTK4174] - isotype control (ab114134)
  • Recombinant Protein

    • Recombinant human CD3 epsilon protein (Active) (ab167755)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab24948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 3µg for 106 cells.

In vitro depletion of CD3+ cells and activation of T cells (accessory cells must be provided).
ab114134 - Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.

Notes
Flow Cyt
Use 3µg for 106 cells.

In vitro depletion of CD3+ cells and activation of T cells (accessory cells must be provided).
ab114134 - Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.

Target

  • Function

    The CD3 complex mediates signal transduction.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P07766 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12501 Mouse
    • SwissProt: P22646 Mouse
    • Unigene: 210361 Mouse
    • Alternative names

      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    Images

    • Flow Cytometry - FITC Anti-CD3 epsilon antibody [C363.29B] (ab24948)
      Flow Cytometry - FITC Anti-CD3 epsilon antibody [C363.29B] (ab24948)

      Flow Cytometry analysis of C57BL/6 mouse splenocytes stained with Anti-CD3 epsilon antibody [C363.29B] (FITC) ab24948 (1 µg/106 cells) and Rat Anti-Mouse CD19-PE.

    • Flow Cytometry - FITC Anti-CD3 epsilon antibody [C363.29B] (ab24948)
      Flow Cytometry - FITC Anti-CD3 epsilon antibody [C363.29B] (ab24948)
      Ab24948 (3µg for 106 cells) and anti mouse CD45R/B220 RPE conjugate antibody doubly staining cells from BALB/c mesenteric lymph nodes, gated on small lymphocytes and analyzed using a flow cytometer.

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab24948? Please let us know so that we can cite the reference in this datasheet.

    ab24948 has been referenced in 3 publications.

    • Mohamad NE  et al. Apoptosis and metastasis inhibitory potential of pineapple vinegar against mouse mammary gland cells in vitro and in vivo. Nutr Metab (Lond) 16:49 (2019). PubMed: 31372176
    • Zulkawi N  et al. In vitro characterization and in vivo toxicity, antioxidant and immunomodulatory effect of fermented foods; Xeniji™. BMC Complement Altern Med 17:344 (2017). Flow Cyt ; Mouse . PubMed: 28666436
    • Muraille E  et al. Direct visualization of peptide/MHC complexes at the surface and in the intracellular compartments of cells infected in vivo by Leishmania major. PLoS Pathog 6:e1001154 (2010). PubMed: 20976202

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a question

    Question

    Purchased wrong antibody ab18703 and ab18450. How could we return the antibodies? We are interested in getting ab18446 and rat monoclonal anti IgG2c antibody which we could not find in catalogue.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 24 2011

    Answer

    Thank you for your phone call. I can confirm that you can send the products back to us. When you return the goods, please ensure that you follow the procedures as below: - Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today. - Return all documents (or copies of) that arrived with the product (if they’re available – should be invoice, delivery note and datasheet). Please note that we will be unable to issue the credit note if the product is dispatched without the cooler box. However we are not able to accept products returned on dry ice. - Please mark on the documents the reason of return and Ref: - Please return using an over night courier. - You will need to provide the courier with an AirWay Bill – which you can get from the courier, and fill out with our details. - Our address is: Abcam plc, 330 Cambridge Science Park, Milton Road, Cambridge, CB4 0FL When we have received the goods, we will process the return. We will then raise a credit note for the invoice less 20% restocking fee. If you have already paid this invoice, we will reallocate this money to a new invoice for a future order and if there’s any difference in price, we will refund or re-invoice accordingly. For the correct item, please place a new order in the normal methods. Please confirm if you are planning to return the product to us. Important: if the product is not returned to Abcam within next 7 days we will cancel the credit note. PS: We have anti IgG2a and anti IgG2c available in catalogue that are currently in stock. These 2 antibodies exactly match your request. The catalogue numbers are: ab18446 and ab114134

    Read More

    Abcam Scientific Support

    Answered on Aug 24 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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