Recombinant FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)
![Immunocytochemistry - FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)](https://www.abcam.com/ps/products/237/ab237453/Images/ab237453-398935-AntiCD3-zeta-phospho-Y83-antibody-EP7762Y-FITC-immunocytochemistry-jurkat-human.jpg "Immunocytochemistry - FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- FITC Rabbit monoclonal [EP776(2)Y] to CD3 zeta (phospho Y83)
- Suitable for: ICC, Flow Cyt
- Conjugation: FITC. Ex: 493nm, Em: 528nm
Overview
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Product name
FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]
See all CD3 zeta primary antibodies -
Description
FITC Rabbit monoclonal [EP776(2)Y] to CD3 zeta (phospho Y83) -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
This antibody detects CD3 zeta phosphorylated on tyrosine 83. -
Tested applications
Suitable for: ICC, Flow Cytmore details -
Immunogen
Synthetic peptide within Human CD3 zeta aa 50-150 (phospho Y83). The exact sequence is proprietary.
Database link: P20963 -
Positive control
- ICC: Pervanadate treated Jurkat cells. Flow Cyt: Pervanadate treated Jurkat cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EP776(2)Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab237453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. |
Target
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Function
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. -
Involvement in disease
Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. -
Sequence similarities
Belongs to the CD3Z/FCER1G family.
Contains 3 ITAM domains. -
Domain
The ITAM domains mediate interaction with SHB. -
Post-translational
modificationsPhosphorylated on Tyr residues after T-cell receptor triggering. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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Immunocytochemistry - FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)
ab237453 staining CD3 zeta (phospho Y83) in Jurkat cells +/- pervanadate ( 1mM, 30min). The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, then incubated ab237453 at 1/50 dilution (shown in green). Counterstained with ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
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![Flow Cytometry - FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)](https://www.abcam.com/ps/products/237/ab237453/Images/ab237453-398934-AntiCD3-zeta-phospho-Y83-antibody-EP7762Y-FITC-flow-cytometry-jurkat-human.jpg)
Flow Cytometry - FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)Flow cytometry overlay histogram showing Jurkat + pervanadate ( 1mM, 30min)(Red) / Untreated control (Green) stained with ab237453. The cells were fixed with 4 % formaldehyde (10 min). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab237453).
Isotype control antibody (Black) was Rabbit IgG, monoclonal [EPR25A] - Isotype Control (FITC) (ab223339) used at the same concentration and conditions as the primary antibody.
Unlabelled sample without incubation with primary antibody and secondary antibody (Blue) was also used as a control.
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![FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)](https://www.abcam.com/ps/products/237/ab237453/Images/ab237453-3-benefits-of-recombinant-antibodies.png)
FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab237453 has not yet been referenced specifically in any publications.
