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    fitc-cd31-antibody-b-b38-ab27333.pdf

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FITC Anti-CD31 antibody [B-B38] (ab27333)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (6)

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Abpromise

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Flow Cytometry - FITC Anti-CD31 antibody [B-B38] (ab27333)

    Key features and details

    • FITC Mouse monoclonal [B-B38] to CD31
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant human CD31 protein (ab155607)

    View more associated products

    Overview

    • Product name

      FITC Anti-CD31 antibody [B-B38]
      See all CD31 primary antibodies
    • Description

      FITC Mouse monoclonal [B-B38] to CD31
    • Host species

      Mouse
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant full length CD31 protein (Human).

    • General notes

      This product was changed from ascites to tissue culture supernatant on 19th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.3
      Preservative: 0.1% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Purification notes

      Conjugated from an Affinity purified culture supernatant.
    • Clonality

      Monoclonal
    • Clone number

      B-B38
    • Myeloma

      x63-Ag8.653
    • Isotype

      IgG1
    • Research areas

      • Cardiovascular
      • Blood
      • Platelets
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Cell Adhesion Molecules
      • Endothelial
      • Cardiovascular
      • Angiogenesis
      • Angiogenic Factors
      • Stem Cells
      • Mesenchymal Stem Cells
      • Surface Molecules
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Cell adhesion
      • Other
      • Cancer
      • Tumor immunology
      • CD markers
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Leukocyte recruitment
      • Cell adhesion molecules
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Neutrophil Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Thrombocytic Lineage
      • Cardiovascular
      • Cardiovascular Markers
      • Cell Markers
      • Endothelial Cells
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    Associated products

    • Alternative Versions

      • Anti-CD31 antibody [B-B38] (ab46922)
    • Isotype control

      • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
    • Recombinant Protein

      • Recombinant human CD31 protein (ab155607)

    Applications

    Our Abpromise guarantee covers the use of ab27333 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
    • Tissue specificity

      Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
    • Sequence similarities

      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    • Domain

      The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
    • Post-translational
      modifications

      Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
    • Cellular localization

      Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
    • Target information above from: UniProt accession P16284 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5175 Human
      • Omim: 173445 Human
      • SwissProt: P16284 Human
      • Unigene: 376675 Human
      • Unigene: 514412 Human
      • Alternative names

        • Adhesion molecule antibody
        • CD31 antibody
        • CD31 antigen antibody
        • CD31 EndoCAM antibody
        • EndoCAM antibody
        • FLJ34100 antibody
        • FLJ58394 antibody
        • GPIIA antibody
        • GPIIA' antibody
        • PECA1 antibody
        • PECA1_HUMAN antibody
        • Pecam 1 antibody
        • PECAM 1 CD31 EndoCAM antibody
        • PECAM antibody
        • PECAM-1 antibody
        • Pecam1 antibody
        • Platelet and endothelial cell adhesion molecule 1 antibody
        • Platelet endothelial cell adhesion molecule antibody
        • Platelet/endothelial cell adhesion molecule 1 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-CD31 antibody [B-B38] (ab27333)
        Flow Cytometry - FITC Anti-CD31 antibody [B-B38] (ab27333)

        Ab27333, staining CD31 by FACS.

        This image was generated using the ascites version of the product.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (6)

      Publishing research using ab27333? Please let us know so that we can cite the reference in this datasheet.

      ab27333 has been referenced in 6 publications.

      • Karakas N  et al. Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity. PLoS One 15:e0228510 (2020). PubMed: 32407317
      • Sikura KÉ  et al. Potential Role of H-Ferritin in Mitigating Valvular Mineralization. Arterioscler Thromb Vasc Biol 39:413-431 (2019). PubMed: 30700131
      • Zhu L  et al. Spermine on Endothelial Extracellular Vesicles Mediates Smoking-Induced Pulmonary Hypertension Partially Through Calcium-Sensing Receptor. Arterioscler Thromb Vasc Biol 39:482-495 (2019). PubMed: 30626206
      • Avril M  et al. Interaction between Endothelial Protein C Receptor and Intercellular Adhesion Molecule 1 to Mediate Binding of Plasmodium falciparum-Infected Erythrocytes to Endothelial Cells. MBio 7:N/A (2016). Flow Cyt ; Human . PubMed: 27406562
      • Yuan SM  et al. PDGFR-ß (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo. Int J Clin Exp Pathol 7:2861-70 (2014). Flow Cyt ; Human . PubMed: 25031705
      • Pain A  et al. Platelet-mediated clumping of Plasmodium falciparum-infected erythrocytes is a common adhesive phenotype and is associated with severe malaria. Proc Natl Acad Sci U S A 98:1805-10 (2001). PubMed: 11172032

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      Sorry for forgetting to ask another question. I noted that the application of some antibodies is only for flow cytometry.CouldI use these antibodies to do western blot orICC? Thank you!

      Read More

      Abcam community

      Verified customer

      Asked on Jan 16 2012

      Answer

      If the antibody datasheets only list "flow cytometry" in the Applications section, then that is the only application that has been tested. It is possible that the antibodies could also work for western blotting or ICC, but these applications have not been tested, and they are not covered by our guarantee.

      Read More

      Abcam Scientific Support

      Answered on Jan 16 2012

      Question

      For flow cytometry, people always use normal cell(without antibody disposal) as control to set up the machine before running. But there are also some people use isotype antibody to replace normal cell as control. Which method is recommended for my control group to set up program?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 14 2012

      Answer

      A cells-only control with no antibody will show the contribution of autofluorescence of the cells themselves. Most people use this to set up the flow cytometer. An isotype control (cells incubated with the same isotype of antibody as the target-specific antibody) will include autofluorescence signal but also the non-epitope-specific interaction of the antibody with the samples. Examples of these interactions include the binding of the Fc region of the antibody to Fc receptors on the cells, and any non-epitope-specific antibody-protein interactions (for instance, hydrophobic interactions) . You could use this isotype control to adjust your flow cytomer settings but most people include it as an experimental control, after setting up the flow cytometer with cells-only. You will be able to see if there is any signal from the isotype interactions by comparing the isotype control to the cells-only control. The antibodies conjugated to FITC that you asked about are all isotype IgG1 (ab30437, ab27333, ab11155). An appropriate isotype control is ab106163: Click here (or use the following: https://www.abcam.com/index.html?datasheet=106163).

      Read More

      Abcam Scientific Support

      Answered on Jan 14 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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