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  1. Link

    fitc-cd32b--cd32a-antibody-at10-ab30356.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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FITC Anti-CD32B + CD32A antibody [AT10] (ab30356)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - FITC Anti-CD32B + CD32A antibody [AT10] (ab30356)
  • Flow Cytometry - FITC Anti-CD32B + CD32A antibody [AT10] (ab30356)

Key features and details

  • FITC Mouse monoclonal [AT10] to CD32B + CD32A
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

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Conjugation
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FITC Conjugation Kit - Lightning-Link® (ab102884)
Protein
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Recombinant human CD32A protein (ab207140)

View more associated products

Overview

  • Product name

    FITC Anti-CD32B + CD32A antibody [AT10]
  • Description

    FITC Mouse monoclonal [AT10] to CD32B + CD32A
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CD32A.

  • Positive control

    • Flow Cyt: K562 cells. ICC/IF: K562 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 30% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    AT10
  • Myeloma

    NS1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Immunoglobulins
    • Receptors
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Mast Cell Lineage
    • Immunology
    • Innate Immunity
    • Fc Receptors

Associated products

  • Alternative Versions

    • Anti-CD32A+CD32B antibody [AT10] (ab23336)
    • Biotin Anti-CD32B + CD32A antibody [AT10] (ab30355)
    • PE Anti-CD32B + CD32A antibody [AT10] (ab30357)
    • Anti-CD32B + CD32A antibody [AT10] - BSA free (ab41899)
  • Isotype control

    • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
  • Recombinant Protein

    • Recombinant human CD32A protein (ab207140)

Applications

Our Abpromise guarantee covers the use of ab30356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

This product gave a positive signal in K562 cells fixed with 4% formaldehyde (10 min) and 80% methanol (5 min).

Flow Cyt 1/500.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Cellular localization

    CD32B: Cell membrane. CD32A: Cell membrane.
  • Database links

    • Entrez Gene: 2212 Human
    • Entrez Gene: 2213 Human
    • Omim: 146790 Human
    • Omim: 604590 Human
    • SwissProt: P12318 Human
    • SwissProt: P31994 Human
    • Unigene: 352642 Human
    • Unigene: 654395 Human
    • Unigene: 713617 Human
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - FITC Anti-CD32B + CD32A antibody [AT10] (ab30356)
    Immunocytochemistry/ Immunofluorescence - FITC Anti-CD32B + CD32A antibody [AT10] (ab30356)

    ab30356 staining CD32A + CD32B in K562 cells. The cells were fixed with 4% formaldehyde (10 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab30356 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in K562 cells fixed with 80% methanol (5 min).

  • Flow Cytometry - FITC Anti-CD32B + CD32A antibody [AT10] (ab30356)
    Flow Cytometry - FITC Anti-CD32B + CD32A antibody [AT10] (ab30356)

    Overlay histogram showing K562 cells stained with ab30356 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab30356, 1/5000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Mouse IgG1 (monoclonal) FITC (ab18435) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (4)

    Publishing research using ab30356? Please let us know so that we can cite the reference in this datasheet.

    ab30356 has been referenced in 4 publications.

    • Tzeng SJ  et al. Fc?RIIB mediates antigen-independent inhibition on human B lymphocytes through Btk and p38 MAPK. J Biomed Sci 22:87 (2015). PubMed: 26475492
    • Lilliehöök I  et al. Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies. Vet Immunol Immunopathol 61:181-93 (1998). PubMed: 9613433
    • Metes D  et al. Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene. Blood 91:2369-80 (1998). PubMed: 9516136
    • Greenman J  et al. Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors. Mol Immunol 28:1243-54 (1991). PubMed: 1835758

    Customer reviews and Q&As

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