FITC Anti-CD33 antibody [WM53] (ab234216)

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Flow Cytometry - FITC Anti-CD33 antibody [WM53] (ab234216)

    Key features and details

    • FITC Mouse monoclonal [WM53] to CD33
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    Overview

    • Product name

      FITC Anti-CD33 antibody [WM53]
      See all CD33 primary antibodies

    • Description

      FITC Mouse monoclonal [WM53] to CD33

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Non human primates

    • Immunogen

      Tissue, cells or virus corresponding to CD33. (Human AML cells).

    • Positive control

      • Flow Cytometry: Human peripheral blood.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab234216 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 4µl for 106 cells.

    (or 100 μL whole blood)

    Target

    • Function

      Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).

    • Tissue specificity

      Monocytic/myeloid lineage cells.

    • Sequence similarities

      Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Domain

      Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

    • Post-translational
      modifications

      Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 33 antibody
        • CD33 antibody
        • CD33 antigen (gp67) antibody
        • CD33 antigen antibody
        • CD33 molecule antibody
        • CD33_HUMAN antibody
        • FLJ00391 antibody
        • gp67 antibody
        • My9 antibody
        • Myeloid cell surface antigen CD33 antibody
        • Myeloid cell surface antigen CD33 precursor antibody
        • Myeloid differentiation antigen CD33 antibody
        • p67 antibody
        • Sialic acid binding Ig like lectin 3 antibody
        • Sialic acid binding immunoglobulin like lectin 3 antibody
        • Sialic acid-binding Ig-like lectin 3 antibody
        • SIGLEC 3 antibody
        • Siglec-3 antibody
        • SIGLEC3 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-CD33 antibody [WM53] (ab234216)
        Flow Cytometry - FITC Anti-CD33 antibody [WM53] (ab234216)

        Flow Cytometry analysis of human peripheral blood labeling CD33 with ab234216. Surface staining. Leukocyte gate.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab234216? Please let us know so that we can cite the reference in this datasheet.

      ab234216 has not yet been referenced specifically in any publications.

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