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  1. Link

    fitc-cd34-antibody-4h11apg-ab18227.pdf

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FITC Anti-CD34 antibody [4H11[APG]] (ab18227)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (5)

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Flow Cytometry - FITC Anti-CD34 antibody [4H11[APG]] (ab18227)

    Key features and details

    • FITC Mouse monoclonal [4H11[APG]] to CD34
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    You may also be interested in

    Protein
    Recombinant Human CD34 protein (ab126924)

    View more associated products

    Overview

    • Product name

      FITC Anti-CD34 antibody [4H11[APG]]
      See all CD34 primary antibodies
    • Description

      FITC Mouse monoclonal [4H11[APG]] to CD34
    • Host species

      Mouse
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.

    • Epitope

      Class III epitope on CD34 antigen.
    • Positive control

      • Human blood cells.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituents: PBS, BSA
    • Concentration information loading...
    • Purification notes

      Purified
    • Clonality

      Monoclonal
    • Clone number

      4H11[APG]
    • Isotype

      IgG1
    • Research areas

      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Soma marker
      • Immunology
      • Cell Type Markers
      • CD
      • Myeloid Cells
      • Stem Cells
      • Lineage Markers
      • Mesoderm
      • Stem Cells
      • Mesenchymal Stem Cells
      • Negative Markers
      • Stem Cells
      • Hematopoietic Progenitors
      • Surface Molecules
      • Cancer
      • Tumor biomarkers
      • Other
      • Cardiovascular
      • Heart
      • Cardiogenesis
      • Stem cells
      • Stem Cells
      • Hematopoietic Progenitors
      • Hematopoietic Stem Cells
      • HSC markers
      • Stem Cells
      • Endothelial Progenitors
      • Endothelial Markers
      • Developmental Biology
      • Lineage specification
      • Mesoderm
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    Associated products

    • Alternative Versions

      • Biotin Anti-CD34 antibody [4H11[APG]] (ab21893)
      • PE/Cy7® Anti-CD34 antibody [4H11APG] (ab233636)
      • Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)
    • Isotype control

      • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
    • Recombinant Protein

      • Recombinant Human CD34 protein (ab126924)

    Applications

    Our Abpromise guarantee covers the use of ab18227 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
  • Application notes
    Flow Cyt: Use 20 µl reagent / 100 µl sample.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
    • Tissue specificity

      Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
    • Sequence similarities

      Belongs to the CD34 family.
    • Developmental stage

      On early hematopoietic progenitor cells.
    • Post-translational
      modifications

      Highly glycosylated.
      Phosphorylated on serine residues by PKC.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P28906 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 947 Human
      • Omim: 142230 Human
      • SwissProt: P28906 Human
      • Unigene: 374990 Human
      • Alternative names

        • CD34 antibody
        • CD34 antigen antibody
        • CD34 molecule antibody
        • CD34_HUMAN antibody
        • Cluster designation 34 antibody
        • cluster of differentiation 34 antibody
        • Hematopoietic progenitor cell antigen CD34 antibody
        • HPCA1 antibody
        • Mucosialin antibody
        • OTTHUMP00000034733 antibody
        • OTTHUMP00000034734 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-CD34 antibody [4H11[APG]] (ab18227)
        Flow Cytometry - FITC Anti-CD34 antibody [4H11[APG]] (ab18227)

        Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC (ab18227). Total viable cells were used for analysis.

        ab18227 was used at a recommended dilution of 2 μg/ml.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (5)

      Publishing research using ab18227? Please let us know so that we can cite the reference in this datasheet.

      ab18227 has been referenced in 5 publications.

      • Karakas N  et al. Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity. PLoS One 15:e0228510 (2020). PubMed: 32407317
      • Huang N  et al. Neuroprotective effect of tanshinone IIA-incubated mesenchymal stem cells on Aß25-35-induced neuroinflammation. Behav Brain Res 365:48-55 (2019). PubMed: 30831140
      • Wu YT  et al. A novel and feasible way to cultivate and purify endothelial progenitor cells from bone marrow of children with congenital heart diseases. Chin Med J (Engl) 125:1903-7 (2012). Flow Cyt ; Human . PubMed: 22884051
      • Sampat SR  et al. Growth factor priming of synovium-derived stem cells for cartilage tissue engineering. Tissue Eng Part A 17:2259-65 (2011). Flow Cyt ; Cow . PubMed: 21542714
      • Gong Z & Niklason LE Small-diameter human vessel wall engineered from bone marrow-derived mesenchymal stem cells (hMSCs). FASEB J 22:1635-48 (2008). Flow Cyt ; Human . PubMed: 18199698

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      What is the IgG concentration of ab18227? I want to match the concentration of an isotype control for flow cytometry.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 08 2012

      Answer

      Thank you for contacting us. The concentration of ab18227, anti-CD34, has been determined and is 50 ug/ml for the lot that you have. So, 20 ul contains 1 ug.

      Abcam also carries other products essential for Flow Cytometry such as isotype controls. If testing live cells we also recommend cell viability controls such as 7-AAD or Propidium Iodide. Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AAD Cell-Mediated Cytotoxicity Assay Kit.

      Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html

      7-AAD Kit: https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html

      I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

      Read More

      Abcam Scientific Support

      Answered on Nov 08 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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