FITC Anti-CD34 antibody [581] (ab131589)
![Flow Cytometry - FITC Anti-CD34 antibody [581] (ab131589)](https://www.abcam.com/ps/products/131/ab131589/Images/ab131589-1-anti-human-CD34-fitc-flow-cyt-human-peripheral-blood.jpg "Flow Cytometry - FITC Anti-CD34 antibody [581] (ab131589)")
Key features and details
- FITC Mouse monoclonal [581] to CD34
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD34 antibody [581]
See all CD34 primary antibodies -
Description
FITC Mouse monoclonal [581] to CD34 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab131589 recognizes the class III CD34 epitope resistant to neuraminidase, chymopapain and glycoprotease. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Epitope
An extracellular epitope of CD34. -
Positive control
- Flow Cyt: Human blood cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 0.2% BSA, 99% PBS -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
581 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab131589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
Target
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Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
Sequence similarities
Belongs to the CD34 family. -
Developmental stage
On early hematopoietic progenitor cells. -
Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
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Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Images
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Flow Cytometry - FITC Anti-CD34 antibody [581] (ab131589)Flow cytometry surface staining pattern of human peripheral whole blood labeling CD34 with ab131589 (4 µL reagent/100 µL of peripheral whole blood).
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![Flow Cytometry - FITC Anti-CD34 antibody [581] (ab131589)](https://www.abcam.com/ps/products/131/ab131589/Images/ab131589-3-anti-cd34-fitc-antibody-flow-cyt-cd34-positive-stem-cells-human.jpg)
Flow Cytometry - FITC Anti-CD34 antibody [581] (ab131589)Flow cytometry analysis (surface staining) of human peripheral whole blood labeling CD34 with ab131589 (4 µL reagent/100 µL of peripheral whole blood). Histogram showing separation of human CD34 positive stem cells (red-filled) from lymphocytes (black-dashed). -
![Flow Cytometry - FITC Anti-CD34 antibody [581] (ab131589)](https://www.abcam.com/ps/products/131/ab131589/Images/ab131589-2-anti-human-CD34-fitc-flow-cyt-human-peripheral-blood-2.jpg)
Flow Cytometry - FITC Anti-CD34 antibody [581] (ab131589)Flow cytometry surface staining pattern of human peripheral whole blood showing CD34 positive stem cells (red) stained using ab131589 (4 µL reagent/100 µL of peripheral whole blood).
References (1)
ab131589 has been referenced in 1 publication.
- Lv YJ et al. Low-dose cadmium exposure acts on rat mesenchymal stem cells via RANKL/OPG and downregulate osteogenic differentiation genes. Environ Pollut 249:620-628 (2019). PubMed: 30933759
