FITC Anti-CD34 antibody [QBEND/10] (ab78165)
![Flow Cytometry - FITC Anti-CD34 antibody [QBEND/10] (ab78165)](https://www.abcam.com/ps/products/78/ab78165/Images/ab78165-93962-anti-cd34-antibody-qbend-10-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD34 antibody [QBEND/10] (ab78165)")
Key features and details
- FITC Mouse monoclonal [QBEND/10] to CD34
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
FITC Anti-CD34 antibody [QBEND/10]
See all CD34 primary antibodies -
Description
FITC Mouse monoclonal [QBEND/10] to CD34 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab78165 recognizes CD34, a heavily glycosylated transmembrane protein, gp 105-120 kDa, expressed on immature human haemopoietic precursor cells, capillary endothelial cells and human myeloid cells in the following categories: subsets of CFU-GEMM and BFU-E, all CFU-GM, granulocyte precursors, monocytes, myeloid leukaemias. ab78165 recognizes the class II epitope showing resistance to neuraminidase enzyme cleavage. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Does not react with: Rat, Sheep, Cow, Dog -
Immunogen
Tissue, cells or virus corresponding to Human CD34. Human endothelial vesicles
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Positive control
- KG-1 cell line
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
QBEND/10 -
Myeloma
NS0 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (1) |
| Notes |
|---|
Application notes
Flow Cyt: Use 10µl per test.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
Sequence similarities
Belongs to the CD34 family. -
Developmental stage
On early hematopoietic progenitor cells. -
Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
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Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Images
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Flow Cytometry - FITC Anti-CD34 antibody [QBEND/10] (ab78165)Log fluorescence intensity profile of KG-1 cell line stained with ab78165 at 10ul per test.
Datasheets and documents
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Datasheet download
References (7)
ab78165 has been referenced in 7 publications.
- Wei X et al. Ultrasound-targeted microbubble destruction-mediated Galectin-7-siRNA promotes the homing of bone marrow mesenchymal stem cells to alleviate acute myocardial infarction in rats. Int J Mol Med 47:677-687 (2021). PubMed: 33416139
- Ou X et al. Activation of SIRT1 promotes cartilage differentiation and reduces apoptosis of nucleus pulposus mesenchymal stem cells via the MCP1/CCR2 axis in subjects with intervertebral disc degeneration. Int J Mol Med 46:1074-1084 (2020). PubMed: 32705163
- Wu D et al. Long noncoding RNA TUG1 promotes osteogenic differentiation of human periodontal ligament stem cell through sponging microRNA-222-3p to negatively regulate Smad2/7. Arch Oral Biol 117:104814 (2020). PubMed: 32574885
- Sayilekshmy M et al. Innervation is higher above Bone Remodeling Surfaces and in Cortical Pores in Human Bone: Lessons from patients with primary hyperparathyroidism. Sci Rep 9:5361 (2019). PubMed: 30926835
- Montazersaheb S et al. Prolonged incubation with Metformin decreased angiogenic potential in human bone marrow mesenchymal stem cells. Biomed Pharmacother 108:1328-1337 (2018). PubMed: 30372835
- Rotolo A et al. Enhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting. Cancer Cell 34:596-610.e11 (2018). PubMed: 30300581
- Jensen PR et al. Premature loss of bone remodeling compartment canopies is associated with deficient bone formation: a study of healthy individuals and patients with Cushing's syndrome. J Bone Miner Res 27:770-80 (2012). PubMed: 22162180
