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    fitc-cd34-antibody-qbend10-ab78165.pdf

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FITC Anti-CD34 antibody [QBEND/10] (ab78165)

  • Datasheet
Reviews (1)Q&A (2)References (5)

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Abpromise

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Flow Cytometry - FITC Anti-CD34 antibody [QBEND/10] (ab78165)

    Key features and details

    • FITC Mouse monoclonal [QBEND/10] to CD34
    • Suitable for: Flow Cyt
    • Reacts with: Human, Cynomolgus monkey, Rhesus monkey
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

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    Overview

    • Product name

      FITC Anti-CD34 antibody [QBEND/10]
      See all CD34 primary antibodies
    • Description

      FITC Mouse monoclonal [QBEND/10] to CD34
    • Host species

      Mouse
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Specificity

      ab78165 recognizes CD34, a heavily glycosylated transmembrane protein, gp 105-120 kDa, expressed on immature human haemopoietic precursor cells, capillary endothelial cells and human myeloid cells in the following categories: subsets of CFU-GEMM and BFU-E, all CFU-GM, granulocyte precursors, monocytes, myeloid leukaemias. ab78165 recognizes the class II epitope showing resistance to neuraminidase enzyme cleavage.
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human, Cynomolgus monkey, Rhesus monkey
      Does not react with: Rat, Sheep, Cow, Dog
    • Immunogen

      Human endothelial vesicles

    • Positive control

      • KG-1 cell line
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Preservative: 0.065% Sodium azide
      Constituents: 0.1% BSA, PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      QBEND/10
    • Myeloma

      NS0
    • Isotype

      IgG1
    • Research areas

      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Soma marker
      • Immunology
      • Cell Type Markers
      • CD
      • Myeloid Cells
      • Stem Cells
      • Lineage Markers
      • Mesoderm
      • Stem Cells
      • Mesenchymal Stem Cells
      • Negative Markers
      • Stem Cells
      • Hematopoietic Progenitors
      • Surface Molecules
      • Cancer
      • Tumor biomarkers
      • Other
      • Cardiovascular
      • Heart
      • Cardiogenesis
      • Stem cells
      • Stem Cells
      • Hematopoietic Progenitors
      • Hematopoietic Stem Cells
      • HSC markers
      • Stem Cells
      • Endothelial Progenitors
      • Endothelial Markers
      • Developmental Biology
      • Lineage specification
      • Mesoderm
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    Associated products

    • Alternative Versions

      • Anti-CD34 antibody [QBEnd/10] - BSA and Azide free (ab213054)
    • Isotype control

      • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
    • Recombinant Protein

      • Recombinant Human CD34 protein (ab126924)

    Applications

    Our Abpromise guarantee covers the use of ab78165 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
  • Application notes
    Flow Cyt: Use 10µl per test.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
    • Tissue specificity

      Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
    • Sequence similarities

      Belongs to the CD34 family.
    • Developmental stage

      On early hematopoietic progenitor cells.
    • Post-translational
      modifications

      Highly glycosylated.
      Phosphorylated on serine residues by PKC.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P28906 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 101866926 Cynomolgus monkey
      • Entrez Gene: 947 Human
      • Entrez Gene: 713858 Rhesus monkey
      • Omim: 142230 Human
      • SwissProt: P28906 Human
      • Unigene: 374990 Human
      • Alternative names

        • CD34 antibody
        • CD34 antigen antibody
        • CD34 molecule antibody
        • CD34_HUMAN antibody
        • Cluster designation 34 antibody
        • cluster of differentiation 34 antibody
        • Hematopoietic progenitor cell antigen CD34 antibody
        • HPCA1 antibody
        • Mucosialin antibody
        • OTTHUMP00000034733 antibody
        • OTTHUMP00000034734 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-CD34 antibody [QBEND/10] (ab78165)
        Flow Cytometry - FITC Anti-CD34 antibody [QBEND/10] (ab78165)
        Log fluorescence intensity profile of KG-1 cell line stained with ab78165 at 10ul per test.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (5)

      Publishing research using ab78165? Please let us know so that we can cite the reference in this datasheet.

      ab78165 has been referenced in 5 publications.

      • Ou X  et al. Activation of SIRT1 promotes cartilage differentiation and reduces apoptosis of nucleus pulposus mesenchymal stem cells via the MCP1/CCR2 axis in subjects with intervertebral disc degeneration. Int J Mol Med 46:1074-1084 (2020). PubMed: 32705163
      • Sayilekshmy M  et al. Innervation is higher above Bone Remodeling Surfaces and in Cortical Pores in Human Bone: Lessons from patients with primary hyperparathyroidism. Sci Rep 9:5361 (2019). PubMed: 30926835
      • Montazersaheb S  et al. Prolonged incubation with Metformin decreased angiogenic potential in human bone marrow mesenchymal stem cells. Biomed Pharmacother 108:1328-1337 (2018). PubMed: 30372835
      • Rotolo A  et al. Enhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting. Cancer Cell 34:596-610.e11 (2018). PubMed: 30300581
      • Jensen PR  et al. Premature loss of bone remodeling compartment canopies is associated with deficient bone formation: a study of healthy individuals and patients with Cushing's syndrome. J Bone Miner Res 27:770-80 (2012). PubMed: 22162180

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-3 of 3 Abreviews or Q&A

      Question

      I have a question concerning the above mentioned product. We could not find the concentration of the antibody within the mentioned product. The product information sheet says 10microliter/test. For reasons of comparability, could you please let us know the concentration of the fluorescent antibody.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 08 2012

      Answer

      Thank you for contacting us.

      The concentration of ab78165 is around 100 ug/ml however, the exact concentration is not known.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Nov 08 2012

      Question

      Bonjour,
      Merci pour votre mail.
      Je veux bien un devis pour le cd34 fitc et le cd166 non-couplé.
      bonne journée,

      Read More

      Abcam community

      Verified customer

      Asked on Jan 31 2012

      Answer

      Comme convenu je vous ai fait parvenir un devis pourune unité d'anti-CD34 (FITC) https://www.abcam.com/ab78165 et une unité d'anti-CD166 https://www.abcam.com/ab78649.

      Je me permets de vous rappeller que nous offrons jusqu'au 2 mars 2012 une réduction de 15% sur tous les"nouveaux produits" de notre catalogue, y compris les kits de couplage EasyLink. Le code promotionnel à mentionner sur votre bon de commande pour ces "nouveaux produits" est : FR-NEW-Y7TJP.

      Cette offre est valable pour les produits suivants :

      Kits de conjugaison EasyLink
      Kits ELISA
      Anticorps Secondaires
      Protéines et peptides
      Kits d'immunohistochimie
      Kits de dosage : apoptose, métabolisme, épigénétique

      N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

      Read More

      Abcam Scientific Support

      Answered on Jan 31 2012

      Flow Cytometry abreview for Anti-CD34 antibody [QBEND/10] (FITC)

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Human Cell (KG-1 cells (human acute myeloid leukemia cell line)
      Specification
      KG-1 cells (human acute myeloid leukemia cell line
      Preparation
      Cell harvesting/tissue preparation method: Cells grown in suspension in RPMI-1640 and centrifuged before staining
      Sample buffer: FACS buffer (PBS 0.5% FCS 0.5M EDTA)
      Fixation
      None
      Permeabilization
      No
      Gating Strategy
      All live cells
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 01 2010

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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