Key features and details
- FITC Rat monoclonal [3/23] to CD40
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Product nameFITC Anti-CD40 antibody [3/23]
See all CD40 primary antibodies
DescriptionFITC Rat monoclonal [3/23] to CD40
ConjugationFITC. Ex: 493nm, Em: 528nm
SpecificitySpecificity was checked by ELISA and flow cytometry using BHK cells transfected with mouse CD40. It does not react with normal mouse Ig or with human Ig1 and will stain most mature mouse B cells. It dos not cross react with mouse T cells. Clone 3/23 is a powerful activator of normal B cells especially in the prescence of IL4.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Fusion protein corresponding to Human CD40. Extracellular domain of mouse CD40 and the Fc portion of human IgG1
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesPurified by affinity chromatography on Protein G from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab22470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor for TNFSF5/CD40LG.
Tissue specificityB-cells and in primary carcinomas.
Involvement in diseaseDefects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
Sequence similaritiesContains 4 TNFR-Cys repeats.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
ab22740 staining CD40 in mouse spleen cells by Flow Cytometric analysis.
ab22470 has not yet been referenced specifically in any publications.