FITC Anti-CD40 antibody [HM40-3] (ab95535)
![Flow Cytometry - FITC Anti-CD40 antibody [HM40-3] (ab95535)](https://www.abcam.com/ps/products/95/ab95535/Images/ab95535-109820-anti-cd40-antibody-hm40-3-fitc-flow-cytometry.gif "Flow Cytometry - FITC Anti-CD40 antibody [HM40-3] (ab95535)")
Key features and details
- FITC Armenian hamster monoclonal [HM40-3] to CD40
- Suitable for: Flow Cyt
- Reacts with: Mouse, Rat
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgM
Overview
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Product name
FITC Anti-CD40 antibody [HM40-3]
See all CD40 primary antibodies -
Description
FITC Armenian hamster monoclonal [HM40-3] to CD40 -
Host species
Armenian hamster -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
(BALB/c x NZB) F1 mouse-derived lymphoma WEHI-231 cells.
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Positive control
- Mouse BALB/c splenocytes
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 2.9% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
HM40-3 -
Isotype
IgM -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.5-1µg for 105-8 cells. Staining the cell sample in a final volume of 100µl is recommended. |
Target
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Function
Receptor for TNFSF5/CD40LG. -
Tissue specificity
B-cells and in primary carcinomas. -
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 21939 Mouse
- Entrez Gene: 171369 Rat
- SwissProt: P27512 Mouse
- Unigene: 271833 Mouse
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Alternative names
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
see all
Images
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Flow Cytometry - FITC Anti-CD40 antibody [HM40-3] (ab95535)Flow cytometry staining of BALB/c splenocytes with 0.5 µg of Armenian Hamster IgG Isotype Control FITC (open histogram) or 0.5 µg of ab95535 (filled histogram). Total viable cells were used for analysis.
Datasheets and documents
References (3)
ab95535 has been referenced in 3 publications.
- Ripoll È et al. CD40 gene silencing reduces the progression of experimental lupus nephritis modulating local milieu and systemic mechanisms. PLoS One 8:e65068 (2013). PubMed: 23799000
- Tucsek Z et al. Obesity in Aging Exacerbates Blood-Brain Barrier Disruption, Neuroinflammation, and Oxidative Stress in the Mouse Hippocampus: Effects on Expression of Genes Involved in Beta-Amyloid Generation and Alzheimer's Disease. J Gerontol A Biol Sci Med Sci : (2013). PubMed: 24269929
- Inaba K et al. The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. J Exp Med 180:1849-60 (1994). PubMed: 7525841
