FITC Anti-CD40 antibody [HM40-3] (ab95535)
![Flow Cytometry - FITC Anti-CD40 antibody [HM40-3] (ab95535)](https://www.abcam.com/ps/products/95/ab95535/Images/ab95535-109820-anti-cd40-antibody-hm40-3-fitc-flow-cytometry.gif "Flow Cytometry - FITC Anti-CD40 antibody [HM40-3] (ab95535)")
Key features and details
- FITC Armenian hamster monoclonal [HM40-3] to CD40
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgM
Related conjugates and formulations
Overview
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Product name
FITC Anti-CD40 antibody [HM40-3]
See all CD40 primary antibodies -
Description
FITC Armenian hamster monoclonal [HM40-3] to CD40 -
Host species
Armenian hamster -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD40. (BALB/c x NZB) F1 mouse-derived lymphoma WEHI-231 cells.
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Positive control
- Mouse BALB/c splenocytes
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 2.9% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
HM40-3 -
Isotype
IgM -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 0.5-1µg for 105-8 cells. Staining the cell sample in a final volume of 100µl is recommended.
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| Notes |
|---|
|
Flow Cyt
Use 0.5-1µg for 105-8 cells. Staining the cell sample in a final volume of 100µl is recommended. |
Target
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Function
Receptor for TNFSF5/CD40LG. -
Tissue specificity
B-cells and in primary carcinomas. -
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 21939 Mouse
- SwissProt: P27512 Mouse
- Unigene: 271833 Mouse
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Alternative names
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
see all
Images
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Flow Cytometry - FITC Anti-CD40 antibody [HM40-3] (ab95535)Flow cytometry staining of BALB/c splenocytes with 0.5 µg of Armenian Hamster IgG Isotype Control FITC (open histogram) or 0.5 µg of ab95535 (filled histogram). Total viable cells were used for analysis.
Datasheets and documents
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Datasheet download
References (4)
ab95535 has been referenced in 4 publications.
- Liu S et al. Structural characterization of a novel polysaccharide from Panax notoginseng residue and its immunomodulatory activity on bone marrow dendritic cells. Int J Biol Macromol 161:797-809 (2020). PubMed: 32553971
- Ripoll È et al. CD40 gene silencing reduces the progression of experimental lupus nephritis modulating local milieu and systemic mechanisms. PLoS One 8:e65068 (2013). PubMed: 23799000
- Tucsek Z et al. Obesity in Aging Exacerbates Blood-Brain Barrier Disruption, Neuroinflammation, and Oxidative Stress in the Mouse Hippocampus: Effects on Expression of Genes Involved in Beta-Amyloid Generation and Alzheimer's Disease. J Gerontol A Biol Sci Med Sci : (2013). PubMed: 24269929
- Inaba K et al. The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. J Exp Med 180:1849-60 (1994). PubMed: 7525841
