FITC Anti-CD44 antibody [IM7] (ab218749)
![Flow Cytometry - FITC Anti-CD44 antibody [IM7] (ab218749)](https://www.abcam.com/ps/products/218/ab218749/Images/ab218749-274511-anti-cd44-antibody-im7-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD44 antibody [IM7] (ab218749)")
Key features and details
- FITC Rat monoclonal [IM7] to CD44
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Overview
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Product name
FITC Anti-CD44 antibody [IM7]
See all CD44 primary antibodies -
Description
FITC Rat monoclonal [IM7] to CD44 -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Human
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Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Mouse C57Bl/6 splenocytes.
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General notes
ab218749 may be used for detection of all isoforms of CD44, as it recognizes constant epitopes near the extracellular proximal domain.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, 0.87% Sodium chloride, 0.12% Monobasic dihydrogen sodium phosphate -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab218749 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
IM7 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab218749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. Use 0.5 µg. |
Target
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Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
Sequence similarities
Contains 1 Link domain. -
Domain
The lectin-like LINK domain is responsible for hyaluronan binding. -
Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 960 Human
- Entrez Gene: 12505 Mouse
- Omim: 107269 Human
- SwissProt: P16070 Human
- SwissProt: P15379 Mouse
- Unigene: 502328 Human
- Unigene: 423621 Mouse
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Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
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Flow Cytometry - FITC Anti-CD44 antibody [IM7] (ab218749)
Flow cytometic analysis of mouse C57BI/6 splenocytes labeling CD44 with 0.5 µg ab218749 (solid line) or 0.5 µg rat IgG2b FITC isotype control (dashed line).
Datasheets and documents
References (1)
ab218749 has been referenced in 1 publication.
- Broeckx SY et al. Equine epidermis: a source of epithelial-like stem/progenitor cells with in vitro and in vivo regenerative capacities. Stem Cells Dev 23:1134-48 (2014). PubMed: 24368059
