Key features and details
- FITC Rat monoclonal [KM201] to CD44
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameFITC Anti-CD44 antibody [KM201]
See all CD44 primary antibodies
DescriptionFITC Rat monoclonal [KM201] to CD44
ConjugationFITC. Ex: 493nm, Em: 528nm
SpecificityRecognises all isoforms of CD44/pgp-1 (Mr 80-95 kDa)
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
EpitopeReacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule.
ab25064 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.4
Preservative: 0.1% Sodium azide
Concentration information loading...
Primary antibody notesab25064 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.
Light chain typekappa
Our Abpromise guarantee covers the use of ab25064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 2µg for 106 cells.
ab18404 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Sequence similaritiesContains 1 Link domain.
DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
- Information by UniProt
- LHR antibody
- BA-1 antibody
- CD 44 antibody
ab25064 has been referenced in 4 publications.
- Li G et al. Hypermethylation of microRNA-149 activates SDF-1/CXCR4 to promote osteogenic differentiation of mesenchymal stem cells. J Cell Physiol 234:23485-23494 (2019). PubMed: 31206187
- Hosseinzadeh Shirzeily M et al. Comparison of differentiation potential of male mouse adipose tissue and bone marrow derived-mesenchymal stem cells into germ cells. Iran J Reprod Med 11:965-76 (2013). Flow Cyt ; Mouse . PubMed: 24639722
- Purcell BP et al. Synergistic effects of SDF-1a chemokine and hyaluronic acid release from degradable hydrogels on directing bone marrow derived cell homing to the myocardium. Biomaterials 33:7849-57 (2012). Blocking . PubMed: 22835643
- Fagoonee S et al. Lack of plasma protein hemopexin dampens mercury-induced autoimmune response in mice. J Immunol 181:1937-47 (2008). Flow Cyt ; Mouse . PubMed: 18641331