Key features and details
- FITC Mouse monoclonal [B-A11] to CD45
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameFITC Anti-CD45 antibody [B-A11]
See all CD45 primary antibodies
DescriptionFITC Mouse monoclonal [B-A11] to CD45
ConjugationFITC. Ex: 493nm, Em: 528nm
SpecificityThis antibody is specific for CD45
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to CD45.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.3
Preservative: 0.1% Sodium azide
Constituents: PBS, 5% BSA
Concentration information loading...
PurityIon Exchange Chromatography
Our Abpromise guarantee covers the use of ab27287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
Use 10 µl to label 106 cells or 100 µl of whole blood.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains.
DomainThe first PTPase domain interacts with SKAP1.
modificationsHeavily N- and O-glycosylated.
Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
- B220 antibody
- CD 45 antibody
- CD45 antibody
ab27287 has been referenced in 8 publications.
- Zhang Q et al. Downregulation of antidifferentiation noncoding RNA promotes chondrogenic differentiation and calcification of ligamentum flavum-derived mesenchymal stem cells. J Cell Biochem 120:3401-3414 (2019). PubMed: 30368870
- Kienzle A et al. Free-Floating Mesothelial Cells in Pleural Fluid After Lung Surgery. Front Med (Lausanne) 5:89 (2018). PubMed: 29675416
- Shi Q et al. Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into endometrial cells. Stem Cell Res Ther 8:246 (2017). PubMed: 29096715
- Kim SJ et al. Effect of Bone Marrow Aspirate Concentrate-Platelet-Rich Plasma on Tendon-Derived Stem Cells and Rotator Cuff Tendon Tear. Cell Transplant 26:867-878 (2017). PubMed: 28105983
- Liu YX et al. Attempt towards a novel classification of triple-negative breast cancer using immunohistochemical markers. Oncol Lett 12:1240-1256 (2016). IHC . PubMed: 27446423
- Rossignol J et al. Reductions in behavioral deficits and neuropathology in the R6/2 mouse model of Huntington's disease following transplantation of bone-marrow-derived mesenchymal stem cells is dependent on passage number. Stem Cell Res Ther 6:9 (2015). Mouse . PubMed: 25971780
- Lorenz M et al. Does cellular sex matter? Dimorphic transcriptional differences between female and male endothelial cells. Atherosclerosis 240:61-72 (2015). PubMed: 25756910
- Garcia-Lloret MI et al. Monocytes adhering by LFA-1 to placental syncytiotrophoblasts induce local apoptosis via release of TNF-alpha. A model for hematogenous initiation of placental inflammations. J Leukoc Biol 68:903-8 (2000). PubMed: 11129659