FITC Anti-CD5 antibody [OX-19] (ab24500)

Submit a review Q&A (1)References (2)

Key features and details

  • FITC Mouse monoclonal [OX-19] to CD5
  • Reacts with: Rat
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

Overview

  • Product name

    FITC Anti-CD5 antibody [OX-19]
    See all CD5 primary antibodies

  • Description

    FITC Mouse monoclonal [OX-19] to CD5

  • Host species

    Mouse

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Specificity

    ab24500 recognises CD5 protein.

  • Species reactivity

    Reacts with: Rat

  • Immunogen

    Thymocyte glycoproteins (Rat)

  • General notes

    Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

  • Application notes
    Flow Cyt: Use at an assay dependent dilution.
    IHC-P (tissue fixed with periodate-lysine paraformaldehyde): Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.

    • Sequence similarities

      Contains 3 SRCR domains.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 5 antibody
        • CD5 antibody
        • CD5 antigen (p56 62) antibody
        • CD5 antigen antibody
        • CD5 molecule antibody
        • CD5_HUMAN antibody
        • LEU1 antibody
        • LyA antibody
        • Lymphocyte antigen T1/Leu-1 antibody
        • OTTHUMP00000236973 antibody
        • T-cell surface glycoprotein CD5 antibody
        • T1 antibody
        see all

      References (2)

      Publishing research using ab24500? Please let us know so that we can cite the reference in this datasheet.

      ab24500 has been referenced in 2 publications.

      • Tahta A  et al. Assessment of the MRI and Behavioral Test Results in a Focal Cerebral Ischemia-Reperfusion Model in the Rat after Separate and Combined Use of Mouse-Derived Neural Progenitor Cells, Human-Derived Neural Progenitor Cells and Atorvastatin. Turk Neurosurg 28:571-581 (2018). PubMed: 30192361
      • Whiteland JL  et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-20 (1995). PubMed: 7868861

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Hi,

      I would like to buy antibodies to stain B and T lymphozytes and to differentiate them in monkey blood for Flow Cytometry. I don’t really know what the best antibodies would be to perform such an experiment. Could you help me to find some antibodies?

      Could you also give me a protocol for the staining?
      I look forward hearing from you. I would like to order next week so it would be nice if you could answer as soon as possible.

      Read More
      Answer

      Thank you for contacting us.

      There are many B cell and T cell specific marker, proteins. Please click the link below for more information;

      http://www.antibodybeyond.com/reviews/cell-markers/t-cell-marker.htm

      https://www.abcam.com/Multicolor-Human-B-cell-marker-panel-CD5-PE-CD19-APC-CD20-FITC-and-CD45-PE-Cy7-ab106071.html

      We have antibodies against these markers e.g ab24055, ab27991, ab24500, ab1157, ab1169, ab42941, ab46729 and many more.

      We might not have tested the anti B-cell or T-cell specific antibodies with Monkey cells so you might need to test these your selves. Please use human cells as positive control.

      Please do contact me for more information once you have selected the antibodies.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

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