FITC Anti-CD53 antibody [MEM-53] (ab68565)
![Flow Cytometry - FITC Anti-CD53 antibody [MEM-53] (ab68565)](https://www.abcam.com/ps/products/68/ab68565/Images/ab68565-82847-anti-cd53-antibody-mem-53-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD53 antibody [MEM-53] (ab68565)")
Key features and details
- FITC Mouse monoclonal [MEM-53] to CD53
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD53 antibody [MEM-53]
See all CD53 primary antibodies -
Description
FITC Mouse monoclonal [MEM-53] to CD53 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab68565 reacts with CD53 exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. This antibody reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F). -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Leukocytes of patient suffering from LGL-type leukemia.
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Positive control
- Human blood cells.
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General notes
The antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
MEM-53 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab68565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. Use neat
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
May be involved in growth regulation in hematopoietic cells. -
Tissue specificity
B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 963 Human
- Omim: 151525 Human
- SwissProt: P19397 Human
- Unigene: 443057 Human
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Alternative names
- AI323659 antibody
- Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
- CD 53 antibody
see all
Images
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Flow Cytometry - FITC Anti-CD53 antibody [MEM-53] (ab68565)Overlay histogram showing peripheral blood lymphocytes stained with ab68565 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68565, 1 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.
References (2)
ab68565 has been referenced in 2 publications.
- Rasmussen AM et al. Cross-linking of CD53 promotes activation of resting human B lymphocytes. J Immunol 153:4997-5007 (1994). PubMed: 7963560
- Bazil V et al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35:289-97 (1989). PubMed: 2534480
