FITC Anti-CD63 antibody [MEM-259] (ab18235)
![Flow Cytometry - FITC Anti-CD63 antibody [MEM-259] (ab18235)](https://www.abcam.com/ps/products/18/ab18235/Images/ab18235-27532-anti-cd63-antibody-mem-259-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD63 antibody [MEM-259] (ab18235)")
Key features and details
- FITC Mouse monoclonal [MEM-259] to CD63
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
FITC Anti-CD63 antibody [MEM-259]
See all CD63 primary antibodies -
Description
FITC Mouse monoclonal [MEM-259] to CD63 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD63. T cell line HPB-ALL
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General notes
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
MEM-259 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/5.
20 µl for a 100 µl sample is recommended.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt
1/5. 20 µl for a 100 µl sample is recommended.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
Tissue specificity
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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Database links
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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Alternative names
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
Images
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Flow Cytometry - FITC Anti-CD63 antibody [MEM-259] (ab18235)ab18235 staining CD63 in human peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, analysed by Flow Cytometry.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab18235 has been referenced in 7 publications.
- Slyusarenko M et al. Formation and Evaluation of a Two-Phase Polymer System in Human Plasma as a Method for Extracellular Nanovesicle Isolation. Polymers (Basel) 13:N/A (2021). PubMed: 33572666
- Melo-Baez B et al. MicroRNAs from Extracellular Vesicles Secreted by Bovine Embryos as Early Biomarkers of Developmental Competence. Int J Mol Sci 21:N/A (2020). PubMed: 33255183
- Wang ZF et al. Glioma stem cells-derived exosomal miR-26a promotes angiogenesis of microvessel endothelial cells in glioma. J Exp Clin Cancer Res 38:201 (2019). PubMed: 31101062
- Paik EJ et al. Using intracellular markers to identify a novel set of surface markers for live cell purification from a heterogeneous hIPSC culture. Sci Rep 8:804 (2018). PubMed: 29339826
- Mellisho EA et al. Identification and characteristics of extracellular vesicles from bovine blastocysts produced in vitro. PLoS One 12:e0178306 (2017). Flow Cyt . PubMed: 28542562
- Chen YD et al. Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-?-stimulated lung epithelial cells. Sci Rep 7:5676 (2017). PubMed: 28720835
- Xiao B et al. Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury. Int J Mol Med 40:1201-1209 (2017). PubMed: 28849073