FITC Anti-CD63 antibody [MEM-259] (ab18235)
![Flow Cytometry - FITC Anti-CD63 antibody [MEM-259] (ab18235)](https://www.abcam.com/ps/products/18/ab18235/Images/ab18235-27532-anti-cd63-antibody-mem-259-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD63 antibody [MEM-259] (ab18235)")
Key features and details
- FITC Mouse monoclonal [MEM-259] to CD63
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD63 antibody [MEM-259]
See all CD63 primary antibodies -
Description
FITC Mouse monoclonal [MEM-259] to CD63 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
T cell line HPB-ALL.
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General notes
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
MEM-259 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab18235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/5. 20 µl for a 100 µl sample is recommended.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
Tissue specificity
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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Database links
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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Alternative names
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
Images
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Flow Cytometry - FITC Anti-CD63 antibody [MEM-259] (ab18235)ab18235 staining CD63 in human peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, analysed by Flow Cytometry.
References (5)
ab18235 has been referenced in 5 publications.
- Wang ZF et al. Glioma stem cells-derived exosomal miR-26a promotes angiogenesis of microvessel endothelial cells in glioma. J Exp Clin Cancer Res 38:201 (2019). PubMed: 31101062
- Paik EJ et al. Using intracellular markers to identify a novel set of surface markers for live cell purification from a heterogeneous hIPSC culture. Sci Rep 8:804 (2018). PubMed: 29339826
- Mellisho EA et al. Identification and characteristics of extracellular vesicles from bovine blastocysts produced in vitro. PLoS One 12:e0178306 (2017). Flow Cyt . PubMed: 28542562
- Chen YD et al. Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-?-stimulated lung epithelial cells. Sci Rep 7:5676 (2017). PubMed: 28720835
- Xiao B et al. Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury. Int J Mol Med 40:1201-1209 (2017). PubMed: 28849073
