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  1. Link

    fitc-cd63-antibody-mem-259-ab18235.pdf

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Cardiovascular Blood Platelets
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FITC Anti-CD63 antibody [MEM-259] (ab18235)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (7)

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Flow Cytometry - FITC Anti-CD63 antibody [MEM-259] (ab18235)

    Key features and details

    • FITC Mouse monoclonal [MEM-259] to CD63
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Biotin PE PE/Cy5® PerCP Unconjugated

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    Overview

    • Product name

      FITC Anti-CD63 antibody [MEM-259]
      See all CD63 primary antibodies
    • Description

      FITC Mouse monoclonal [MEM-259] to CD63
    • Host species

      Mouse
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue, cells or virus corresponding to Human CD63. T cell line HPB-ALL

    • General notes

      The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituents: PBS, BSA
    • Concentration information loading...
    • Clonality

      Monoclonal
    • Clone number

      MEM-259
    • Isotype

      IgG1
    • Research areas

      • Cardiovascular
      • Blood
      • Platelets
      • Immunology
      • Cell Type Markers
      • CD
      • Platelets
      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Cell adhesion
      • Other
      • Cancer
      • Tumor biomarkers
      • Tumor antigens

    Associated products

    • Alternative Versions

      • Biotin Anti-CD63 antibody [MEM-259] (ab134331)
      • PE Anti-CD63 antibody [MEM-259], prediluted (ab18236)
      • PE/Cy5® Anti-CD63 antibody [MEM-259] (ab234251)
      • PerCP Anti-CD63 antibody [MEM-259] (ab77227)
      • Anti-CD63 antibody [MEM-259] (ab8219)
    • Isotype control

      • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab18235 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    1/5.

    20 µl for a 100 µl sample is recommended.

     

     

     

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    1/5.

    20 µl for a 100 µl sample is recommended.

     

     

     

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
    • Tissue specificity

      Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
    • Sequence similarities

      Belongs to the tetraspanin (TM4SF) family.
    • Cellular localization

      Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
    • Target information above from: UniProt accession P08962 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 967 Human
      • Omim: 155740 Human
      • SwissProt: P08962 Human
      • Unigene: 445570 Human
      • Alternative names

        • Lysosomal associated membrane protein 3 antibody
        • CD 63 antibody
        • CD63 antibody
        • CD63 antigen (melanoma 1 antigen) antibody
        • CD63 antigen antibody
        • CD63 antigen melanoma 1 antigen antibody
        • CD63 molecule antibody
        • CD63_HUMAN antibody
        • gp55 antibody
        • Granulophysin antibody
        • LAMP 3 antibody
        • LAMP-3 antibody
        • LAMP3 antibody
        • LIMP antibody
        • Lysosomal-associated membrane protein 3 antibody
        • Lysosome associated membrane glycoprotein 3 antibody
        • Lysosome-associated membrane glycoprotein 3 antibody
        • Mast cell antigen AD1 antibody
        • ME491 antibody
        • Melanoma 1 antigen antibody
        • Melanoma associated antigen ME491 antibody
        • Melanoma associated antigen MLA1 antibody
        • Melanoma-associated antigen ME491 antibody
        • MGC72893 antibody
        • MLA 1 antibody
        • MLA1 antibody
        • NGA antibody
        • Ocular melanoma associated antigen antibody
        • Ocular melanoma-associated antigen antibody
        • OMA81H antibody
        • PTLGP40 antibody
        • Tetraspanin 30 antibody
        • Tetraspanin-30 antibody
        • Tspan 30 antibody
        • Tspan-30 antibody
        • TSPAN30 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-CD63 antibody [MEM-259] (ab18235)
        Flow Cytometry - FITC Anti-CD63 antibody [MEM-259] (ab18235)
        ab18235 staining CD63 in human peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, analysed by Flow Cytometry.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (7)

      Publishing research using ab18235? Please let us know so that we can cite the reference in this datasheet.

      ab18235 has been referenced in 7 publications.

      • Slyusarenko M  et al. Formation and Evaluation of a Two-Phase Polymer System in Human Plasma as a Method for Extracellular Nanovesicle Isolation. Polymers (Basel) 13:N/A (2021). PubMed: 33572666
      • Melo-Baez B  et al. MicroRNAs from Extracellular Vesicles Secreted by Bovine Embryos as Early Biomarkers of Developmental Competence. Int J Mol Sci 21:N/A (2020). PubMed: 33255183
      • Wang ZF  et al. Glioma stem cells-derived exosomal miR-26a promotes angiogenesis of microvessel endothelial cells in glioma. J Exp Clin Cancer Res 38:201 (2019). PubMed: 31101062
      • Paik EJ  et al. Using intracellular markers to identify a novel set of surface markers for live cell purification from a heterogeneous hIPSC culture. Sci Rep 8:804 (2018). PubMed: 29339826
      • Mellisho EA  et al. Identification and characteristics of extracellular vesicles from bovine blastocysts produced in vitro. PLoS One 12:e0178306 (2017). Flow Cyt . PubMed: 28542562
      • Chen YD  et al. Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-?-stimulated lung epithelial cells. Sci Rep 7:5676 (2017). PubMed: 28720835
      • Xiao B  et al. Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury. Int J Mol Med 40:1201-1209 (2017). PubMed: 28849073

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Hi, I am just wondering about the estimated molecular weight of ab18235.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 06 2013

      Answer

      The molecular weight of the antibody will be about 150 - 155 kDa, after conjugation with FITC. The molecular weight of CD63 is about 26 kDa, without any post-translational modifications such as glycosylation.

      Read More

      Abcam Scientific Support

      Answered on Feb 06 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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