FITC Anti-CD69 antibody [FN50], prediluted (ab135931)

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Flow Cytometry - FITC Anti-CD69 antibody [FN50], prediluted (ab135931)
  • Flow Cytometry - FITC Anti-CD69 antibody [FN50], prediluted (ab135931)

Key features and details

  • FITC Mouse monoclonal [FN50] to CD69, prediluted
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

Overview

  • Product name

    FITC Anti-CD69 antibody [FN50], prediluted
    See all CD69 primary antibodies

  • Description

    FITC Mouse monoclonal [FN50] to CD69, prediluted

  • Host species

    Mouse

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Anti-µ-stimulated Human B lymphocytes.

  • Positive control

    • Flow Cyt: Human blood cells

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab135931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

20 μl reagent / 100 μl of whole blood or 106 cells in a suspension.

Target

  • Function

    Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

  • Tissue specificity

    Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.

  • Sequence similarities

    Contains 1 C-type lectin domain.

  • Developmental stage

    Earliest inducible cell surface glycoprotein acquired during lymphoid activation.

  • Post-translational
    modifications

    Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Activation inducer molecule (AIM/CD69) antibody
      • Activation inducer molecule antibody
      • AIM antibody
      • BL-AC/P26 antibody
      • BLAC/P26 antibody
      • C-type lectin domain family 2 member C antibody
      • CD69 antibody
      • CD69 antigen (p60, early T-cell activation antigen) antibody
      • CD69 antigen antibody
      • CD69 molecule antibody
      • CD69_HUMAN antibody
      • CLEC2C antibody
      • EA1 antibody
      • Early activation antigen CD69 antibody
      • Early lymphocyte activation antigen antibody
      • Early T cell activation antigen p60 antibody
      • Early T-cell activation antigen p60 antibody
      • GP32/28 antibody
      • Leu23 antibody
      • Leukocyte surface antigen Leu-23 antibody
      • MLR-3 antibody
      • MLR3 antibody
      • VEA antibody
      • Very Early Activation Antigen antibody
      see all

    Images

    • Flow Cytometry - FITC Anti-CD69 antibody [FN50], prediluted (ab135931)
      Flow Cytometry - FITC Anti-CD69 antibody [FN50], prediluted (ab135931)
      Flow cytometry surface staining pattern of human peripheral whole blood stained labeling CD69 with ab135931 (20 µL reagent/100 µL of peripheral whole blood).
    • Flow Cytometry - FITC Anti-CD69 antibody [FN50], prediluted (ab135931)
      Flow Cytometry - FITC Anti-CD69 antibody [FN50], prediluted (ab135931)
      Flow cytometry analysis (surface staining) of human peripheral whole blood labeling CD69 with ab135931 (20 µL reagent/100 µL of peripheral whole blood). Histogram showing separation of human thrombocytes (red-filled) from CD69 negative lymphocytes (black-dashed).

    References (0)

    Publishing research using ab135931? Please let us know so that we can cite the reference in this datasheet.

    ab135931 has not yet been referenced specifically in any publications.

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