FITC Anti-CD69 antibody [FN50], prediluted (ab135931)

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Flow Cytometry - FITC Anti-CD69 antibody [FN50], prediluted (ab135931)
  • Flow Cytometry - FITC Anti-CD69 antibody [FN50], prediluted (ab135931)

Key features and details

  • FITC Mouse monoclonal [FN50] to CD69, prediluted
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    FITC Anti-CD69 antibody [FN50], prediluted
    See all CD69 primary antibodies

  • Description

    FITC Mouse monoclonal [FN50] to CD69, prediluted

  • Host species

    Mouse

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus corresponding to Human CD69. Anti-µ-stimulated Human B lymphocytes.

  • Positive control

    • Flow Cyt: Human blood cells

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab135931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.

20 μl reagent / 100 μl of whole blood or 106 cells in a suspension.
Notes
Flow Cyt
Use at an assay dependent concentration.

20 μl reagent / 100 μl of whole blood or 106 cells in a suspension.

Target

  • Function

    Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

  • Tissue specificity

    Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.

  • Sequence similarities

    Contains 1 C-type lectin domain.

  • Developmental stage

    Earliest inducible cell surface glycoprotein acquired during lymphoid activation.

  • Post-translational
    modifications

    Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Activation inducer molecule (AIM/CD69) antibody
      • Activation inducer molecule antibody
      • AIM antibody
      • BL-AC/P26 antibody
      • BLAC/P26 antibody
      • C-type lectin domain family 2 member C antibody
      • CD69 antibody
      • CD69 antigen (p60, early T-cell activation antigen) antibody
      • CD69 antigen antibody
      • CD69 molecule antibody
      • CD69_HUMAN antibody
      • CLEC2C antibody
      • EA1 antibody
      • Early activation antigen CD69 antibody
      • Early lymphocyte activation antigen antibody
      • Early T cell activation antigen p60 antibody
      • Early T-cell activation antigen p60 antibody
      • GP32/28 antibody
      • Leu23 antibody
      • Leukocyte surface antigen Leu-23 antibody
      • MLR-3 antibody
      • MLR3 antibody
      • VEA antibody
      • Very Early Activation Antigen antibody
      see all

    Images

    • Flow Cytometry - FITC Anti-CD69 antibody [FN50], prediluted (ab135931)
      Flow Cytometry - FITC Anti-CD69 antibody [FN50], prediluted (ab135931)
      Flow cytometry surface staining pattern of human peripheral whole blood stained labeling CD69 with ab135931 (20 µL reagent/100 µL of peripheral whole blood).
    • Flow Cytometry - FITC Anti-CD69 antibody [FN50], prediluted (ab135931)
      Flow Cytometry - FITC Anti-CD69 antibody [FN50], prediluted (ab135931)
      Flow cytometry analysis (surface staining) of human peripheral whole blood labeling CD69 with ab135931 (20 µL reagent/100 µL of peripheral whole blood). Histogram showing separation of human thrombocytes (red-filled) from CD69 negative lymphocytes (black-dashed).

    References (0)

    Publishing research using ab135931? Please let us know so that we can cite the reference in this datasheet.

    ab135931 has not yet been referenced specifically in any publications.

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