FITC Anti-CD79b antibody [AT107-2] (ab33296)
![Flow Cytometry - FITC Anti-CD79b antibody [AT107-2] (ab33296)](https://www.abcam.com/ps/products/33/ab33296/Images/ab33296-46584-anti-cd79b-antibody-at107-2-fitc-flow-cytometry.gif "Flow Cytometry - FITC Anti-CD79b antibody [AT107-2] (ab33296)")
Key features and details
- FITC Rat monoclonal [AT107-2] to CD79b
- Suitable for: Flow Cyt
- Reacts with: Mouse, Rat, Dog, Human, Pig
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD79b antibody [AT107-2]
See all CD79b primary antibodies -
Description
FITC Rat monoclonal [AT107-2] to CD79b -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
Recognises an intracellular region of human CD79b We have data to indicate that this antibody may not cross react with Chicken. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Dog, Human, Pig -
Immunogen
Synthetic peptide corresponding to an intracellular region of human CD79b.
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Positive control
- Rat peripheral blood lymphocytes.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
AT107-2 -
Myeloma
NS1 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab33296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Note: Membrane permeabilisation is required for this application.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation. -
Tissue specificity
B-cells. -
Involvement in disease
Defects in CD79B are the cause of agammaglobulinemia type 6 (AGM6) [MIM:612692]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Post-translational
modificationsPhosphorylated on tyrosine upon B-cell activation. -
Cellular localization
Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts. - Information by UniProt
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Database links
- Entrez Gene: 974 Human
- Entrez Gene: 15985 Mouse
- Entrez Gene: 171055 Rat
- Omim: 147245 Human
- SwissProt: P40259 Human
- SwissProt: P15530 Mouse
- Unigene: 89575 Human
- Unigene: 2987 Mouse
see all -
Alternative names
- AGM6 antibody
- B cell antigen receptor complex associated protein beta chain antibody
- B cell specific glycoprotein B29 antibody
see all
Images
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Flow Cytometry - FITC Anti-CD79b antibody [AT107-2] (ab33296)Staining of rat peripheral blood lymphocytes with ab33296 after permeabilisation.
Datasheets and documents
References (1)
ab33296 has been referenced in 1 publication.
- Sun Z et al. Activated Wnt signaling induces myofibroblast differentiation of mesenchymal stem cells, contributing to pulmonary fibrosis. Int J Mol Med 33:1097-109 (2014). Flow Cyt ; Rat . PubMed: 24573542
