FITC Anti-CD79b antibody [AT107-2] (ab33296)
![Flow Cytometry (Intracellular) - FITC Anti-CD79b antibody [AT107-2] (ab33296)](https://www.abcam.com/ps/products/33/ab33296/Images/ab33296-46584-anti-cd79b-antibody-at107-2-fitc-flow-cytometry.gif "Flow Cytometry (Intracellular) - FITC Anti-CD79b antibody [AT107-2] (ab33296)")
Key features and details
- FITC Rat monoclonal [AT107-2] to CD79b
- Suitable for: Flow Cyt (Intra)
- Reacts with: Rat
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD79b antibody [AT107-2]
See all CD79b primary antibodies -
Description
FITC Rat monoclonal [AT107-2] to CD79b -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
Recognises an intracellular region of human CD79b We have data to indicate that this antibody may not cross react with Chicken. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to an intracellular region of human CD79b.
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Positive control
- Flow Cyt (Intra): Rat peripheral blood lymphocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
AT107-2 -
Myeloma
NS1 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
| Notes |
|---|
Note: Membrane permeabilisation is required for this application.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation. -
Tissue specificity
B-cells. -
Involvement in disease
Defects in CD79B are the cause of agammaglobulinemia type 6 (AGM6) [MIM:612692]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Post-translational
modificationsPhosphorylated on tyrosine upon B-cell activation. -
Cellular localization
Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts. - Information by UniProt
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Database links
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Alternative names
- AGM6 antibody
- B cell antigen receptor complex associated protein beta chain antibody
- B cell specific glycoprotein B29 antibody
see all
Images
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Flow Cytometry (Intracellular) - FITC Anti-CD79b antibody [AT107-2] (ab33296)
Staining of rat peripheral blood lymphocytes with ab33296 after permeabilisation.
Datasheets and documents
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Datasheet download
References (1)
ab33296 has been referenced in 1 publication.
- Sun Z et al. Activated Wnt signaling induces myofibroblast differentiation of mesenchymal stem cells, contributing to pulmonary fibrosis. Int J Mol Med 33:1097-109 (2014). Flow Cyt ; Rat . PubMed: 24573542
