FITC Anti-CD8 alpha antibody [KT15] (ab22504)
Key features and details
- FITC Rat monoclonal [KT15] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Overview
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Product name
FITC Anti-CD8 alpha antibody [KT15]
See all CD8 alpha primary antibodies -
Description
FITC Rat monoclonal [KT15] to CD8 alpha -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab22504 recognises a non-polymorphic epitope on the mouse CD8 alpha chain. Stains mouse Ly2.1 and Ly2.2 cells. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD8 alpha. T cell clone, C6
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General notes
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) FITC : Protein (molar ratio) = 5.3 : 1.0
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
KT15 -
Myeloma
NS0 -
Isotype
IgG2a -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
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Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab22504 has not yet been referenced specifically in any publications.