FITC Anti-CD8 beta antibody [H35-17.2] (ab95773)
![Flow Cytometry - FITC Anti-CD8 beta antibody [H35-17.2] (ab95773)](https://www.abcam.com/ps/products/95/ab95773/Images/ab95773-110015-anti-cd8-beta-antibody-h35-172-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD8 beta antibody [H35-17.2] (ab95773)")
Key features and details
- FITC Rat monoclonal [H35-17.2] to CD8 beta
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Overview
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Product name
FITC Anti-CD8 beta antibody [H35-17.2]
See all CD8 beta primary antibodies -
Description
FITC Rat monoclonal [H35-17.2] to CD8 beta -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c
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Positive control
- BALB/c Mouse splenocytes
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
H35-17.2 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.125-0.25µg for 105-8 cells. A final volume of 100 µL is recommended for staining the cell sample.
ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. -
Tissue specificity
Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPhosphorylated as a consequence of T-cell activation. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12526 Mouse
- SwissProt: P10300 Mouse
- Unigene: 333148 Mouse
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Alternative names
- CD8b antigen antibody
- CD_antigen=CD8b antibody
- CD8 antigen 37 kDa chain antibody
see all
Images
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Flow Cytometry - FITC Anti-CD8 beta antibody [H35-17.2] (ab95773)Staining of BALB/c splenocytes with Anti-Mouse CD8a APC and 0.125 µg of Rat IgG2b ? Isotype Control FITC (left) or ab95773 (right).
Cells in the lymphocyte gate were used for analysis.
Datasheets and documents
References (3)
ab95773 has been referenced in 3 publications.
- Thoma-Uszynski S et al. CD8alphaalpha T cells in lesions of Listeria monocytogenes-infected beta2m-deficient mice. Microb Pathog 23:101-6 (1997). PubMed: 9245621
- Tarleton RL et al. Depletion of T-cell subpopulations results in exacerbation of myocarditis and parasitism in experimental Chagas' disease. Infect Immun 62:1820-9 (1994). PubMed: 8168945
- Lefrancois L Phenotypic complexity of intraepithelial lymphocytes of the small intestine. J Immunol 147:1746-51 (1991). PubMed: 1716278
