FITC Anti-CD9 antibody [MEM-61] (ab18241)
Key features and details
- FITC Mouse monoclonal [MEM-61] to CD9
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
Overview
-
Product name
FITC Anti-CD9 antibody [MEM-61]
See all CD9 primary antibodies -
Description
FITC Mouse monoclonal [MEM-61] to CD9 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD9. Pre-B cell line NALM6
-
Epitope
The antibody recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa protein belonging to tetraspans family (TM4SF). -
Positive control
- Flow Cyt: Human peripheral blood.
-
General notes
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MEM-61 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. |
Notes |
---|
Flow Cyt
Use at an assay dependent concentration. 20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. |
Target
-
Function
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. -
Tissue specificity
Expressed by a variety of hematopoietic and epithelial cells. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsProtein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 928 Human
- Omim: 143030 Human
- SwissProt: P21926 Human
- Unigene: 114286 Human
-
Alternative names
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
see all
Images
-
Flow cytometry surface staining pattern of human peripheral whole blood stained labeling CD9 with ab18241 (20 μl reagent / 100 μl of peripheral whole blood).
-
Flow cytometry surface staining pattern of human peripheral whole blood stained labeling CD9 with ab18241 (20 μl reagent / 100 μl of peripheral whole blood). Histogram showing separation of CD9 positive thrombocytes (red-filled) from CD9 negative lymphocytes (black-dashed).
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab18241 has been referenced in 3 publications.
- Slyusarenko M et al. Formation and Evaluation of a Two-Phase Polymer System in Human Plasma as a Method for Extracellular Nanovesicle Isolation. Polymers (Basel) 13:N/A (2021). PubMed: 33572666
- Jeong H et al. Size-based analysis of extracellular vesicles using sequential transfer of an evaporating droplet. Lab Chip 19:3326-3336 (2019). PubMed: 31497821
- Van Blerkom J & Caltrider K Sperm attachment and penetration competence in the human oocyte: a possible aetiology of fertilization failure involving the organization of oolemmal lipid raft microdomains influenced by the ??m of subplasmalemmal mitochondria. Reprod Biomed Online 27:690-701 (2013). ICC/IF ; Human . PubMed: 24157131