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    fitc-cd90--thy1-antibody-5e10-ab124527.pdf

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Immunology Adaptive Immunity T Cells CD
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FITC Anti-CD90 / Thy1 antibody [5E10] (ab124527)

  • Datasheet
  • SDS
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Flow Cytometry - FITC Anti-CD90 / Thy1 antibody [5E10] (ab124527)

    Key features and details

    • FITC Mouse monoclonal [5E10] to CD90 / Thy1
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

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    FITC Conjugation Kit - Lightning-Link® (ab102884)
    Protein
    Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)

    View more associated products

    Overview

    • Product name

      FITC Anti-CD90 / Thy1 antibody [5E10]
      See all CD90 / Thy1 primary antibodies
    • Description

      FITC Mouse monoclonal [5E10] to CD90 / Thy1
    • Host species

      Mouse
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      HEL erythroleukemia cells.

    • Positive control

      • Flow Cyt: Jurkat cells.
    • General notes

      The reagent is free of unconjugated FITC and adjusted for direct use.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.4
      Preservative: 0.1% Sodium azide
      Constituents: 99% PBS, 0.2% BSA

      BSA is high-grade protease free
    • Concentration information loading...
    • Purity

      Size exclusion
    • Clonality

      Monoclonal
    • Clone number

      5E10
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage

    Associated products

    • Alternative Versions

      • Anti-CD90 / Thy1 antibody [5E10] (ab123511)
      • PerCP/Cy5.5® Anti-CD90 / Thy1 antibody [5E10] (ab134360)
      • Biotin Anti-CD90 / Thy1 antibody [5E10] (ab134361)
      • APC Anti-CD90 / Thy1 antibody [5E10] (ab139364)
      • PE/Cy5® Anti-CD90 / Thy1 antibody [5E10] (ab269286)
      • PE/Cy5® Anti-CD90 / Thy1 antibody [5E10] (ab95698)
      • PE Anti-CD90 / Thy1 antibody [5E10] (ab95700)
    • Isotype control

      • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
    • Recombinant Protein

      • Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)

    Applications

    Our Abpromise guarantee covers the use of ab124527 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    4 μl reagent / 100 μl of whole blood or 106 cells in a suspension.

    Target

    • Function

      May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P04216 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7070 Human
      • Omim: 188230 Human
      • SwissProt: P04216 Human
      • Unigene: 644697 Human
      • Alternative names

        • CD7 antibody
        • CD90 antibody
        • CD90 antigen antibody
        • CDw90 antibody
        • FLJ33325 antibody
        • MGC128895 antibody
        • T25 antibody
        • Theta antigen antibody
        • Thy 1 antibody
        • Thy 1 cell surface antigen antibody
        • Thy 1 membrane glycoprotein antibody
        • Thy 1 T cell antigen antibody
        • Thy 1.2 antibody
        • Thy-1 antigen antibody
        • Thy-1 membrane glycoprotein antibody
        • Thy1 antibody
        • Thy1 antigen antibody
        • Thy1 T cell antigen antibody
        • Thy1.1 antibody
        • Thy1.2 antibody
        • THY1_HUMAN antibody
        • Thymus cell antigen 1, theta antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-CD90 / Thy1 antibody [5E10] (ab124527)
        Flow Cytometry - FITC Anti-CD90 / Thy1 antibody [5E10] (ab124527)
        Flow cytometry analysis (surface staining) using ab124527 (4 µL reagent per milion cells in 100 µL of cell suspension). Histogram showing separation of Jurkat cells (red) from SP2 cells (black).

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (10)

      Publishing research using ab124527? Please let us know so that we can cite the reference in this datasheet.

      ab124527 has been referenced in 10 publications.

      • Conley SM  et al. Human Obesity Induces Dysfunction and Early Senescence in Adipose Tissue-Derived Mesenchymal Stromal/Stem Cells. Front Cell Dev Biol 8:197 (2020). PubMed: 32274385
      • Ou X  et al. Activation of SIRT1 promotes cartilage differentiation and reduces apoptosis of nucleus pulposus mesenchymal stem cells via the MCP1/CCR2 axis in subjects with intervertebral disc degeneration. Int J Mol Med 46:1074-1084 (2020). PubMed: 32705163
      • Wen Y  et al. COL4A2 in the tissue-specific extracellular matrix plays important role on osteogenic differentiation of periodontal ligament stem cells. Theranostics 9:4265-4286 (2019). PubMed: 31285761
      • Pennings I  et al. Effect of donor variation on osteogenesis and vasculogenesis in hydrogel cocultures. J Tissue Eng Regen Med 13:433-445 (2019). PubMed: 30650247
      • Rao F  et al. Exosomes from Human Gingiva-Derived Mesenchymal Stem Cells Combined with Biodegradable Chitin Conduits Promote Rat Sciatic Nerve Regeneration. Stem Cells Int 2019:2546367 (2019). PubMed: 31191669
      • Packthongsuk K  et al. Porcine Wharton's jelly cells distribute throughout the body after intraperitoneal injection. Stem Cell Res Ther 9:38 (2018). PubMed: 29444715
      • Cores J  et al. Safety and Efficacy of Allogeneic Lung Spheroid Cells in a Mismatched Rat Model of Pulmonary Fibrosis. Stem Cells Transl Med 6:1905-1916 (2017). ICC ; Rat . PubMed: 28783251
      • Dinh PC  et al. Derivation of therapeutic lung spheroid cells from minimally invasive transbronchial pulmonary biopsies. Respir Res 18:132 (2017). Flow Cyt . PubMed: 28666430
      • Palazzolo G  et al. Cardiac Niche Influences the Direct Reprogramming of Canine Fibroblasts into Cardiomyocyte-Like Cells. Stem Cells Int 2016:4969430 (2016). PubMed: 26681949
      • Jensen J  et al. Functionalization of polycaprolactone scaffolds with hyaluronic acid and ß-TCP facilitates migration and osteogenic differentiation of human dental pulp stem cells in vitro. Tissue Eng Part A 21:729-39 (2015). PubMed: 25252795

      Customer reviews and Q&As

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