FITC Anti-CD99 antibody [3B2/TA8] (ab130456)
Key features and details
- FITC Mouse monoclonal [3B2/TA8] to CD99
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Overview
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Product name
FITC Anti-CD99 antibody [3B2/TA8]
See all CD99 primary antibodies -
Description
FITC Mouse monoclonal [3B2/TA8] to CD99 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD99. Human thymocytes.
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Positive control
- Flow Cyt: Human blood cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading...
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Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
3B2/TA8 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
Target
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Function
Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes. -
Sequence similarities
Belongs to the CD99 family. -
Post-translational
modificationsExtensively O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4267 Human
- Omim: 313470 Human
- SwissProt: P14209 Human
- Unigene: 653349 Human
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Alternative names
- 12E7 antibody
- Antigen identified by monoclonal 12E7, Y homolog antibody
- Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
see all
Images
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Flow cytometry surface staining pattern of human peripheral whole blood labeling CD99 with ab130456 (4 µL reagent/100 µL of peripheral whole blood).
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Flow cytometry analysis (surface staining) of human peripheral whole blood labeling CD99 with ab130456 (4 µL reagent/100 µL of peripheral whole blood). Histogram showing separation of human CD99 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab130456 has been referenced in 2 publications.
- Pickl WF et al. Lipid rafts and pseudotyping. J Virol 75:7175-83 (2001). PubMed: 11435598
- Waclavicek M et al. CD99 engagement on human peripheral blood T cells results in TCR/CD3-dependent cellular activation and allows for Th1-restricted cytokine production. J Immunol 161:4671-8 (1998). PubMed: 9794396