FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)

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conjugation
  • ab188285 FITC Conjugation Kit used to label mouse anti-sheep CD34 antibody with FITC
  • FITC Conjugation Kit labeling anti MUC1 SP antibodies FACS

Overview

  • Product name

    FITC Conjugation Kit (Fast) - Lightning-Link®
    See all FITC kits

  • Product overview

    FITC Conjugation Kit  / FITC Labeling Kit (Fast) (ab188285) uses a simple and quick process for FITC / Fluorescein labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.


    To conjugate an antibody to FITC / Fluorescein using this kit:
    - add modifier to antibody and incubate for 15 mins
    - add quencher and incubate for 5 mins
    The conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.


    Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to FITC.


    Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

  • Notes

    This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid Fluorescein Labeling Kit. 310-0005 is the same as the 100 ug size. 310-0010 is the same as the 3 x 100 ug size. 310-0030 is the same as the 3 x 10 ug size. 310-0015 is the same as the 1 mg size.

    Amount and volume of antibody for conjugation to FITC

     Kit size Recommended 
    amount of antibody    1     		 Maximum 
    amount of antibody    		 Maximum antibody 
    volume2
    3 x 10 µg 3 x 10 µg  3 x 20 µg 3 x 10 µL
    100 µg 1 x 100 µg 1 x 200 µg 1 x 100µL
    3 x 100 µg 3 x 100 µg  3 x 200 µg 3 x 100 µL
    1 mg 1 x 1 mg 1 x 2 mg 1 x 1 mL

    1 Using the maximum amount of antibody may result in less labelling per antibody. 

    2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/ml or < 0.5 mg/ml should be diluted /concentrated.

     

    Buffer Requirements for Conjugation

    Buffer should be pH 6.5-8.5.

    Compatible buffer constituents 
    If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

    50mM / 0.6% Tris1 0.1% BSA2 50% glycerol
    0.1% sodium azide PBS Potassium phosphate
    Sodium chloride HEPES Sucrose
    Sodium citrate EDTA Trehalose

    1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
    2 BSA can also interfere with the use of the conjugated antibody in tissue staining.

    Incompatible buffer constituents

    Thiomerosal Proclin Glycine
    Arginine Glutathione DTT

    If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

    Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

Properties

Images

  • conjugation
    conjugation
  • ab188285 FITC Conjugation Kit used to label mouse anti-sheep CD34 antibody with FITC
    ab188285 FITC Conjugation Kit used to label mouse anti-sheep CD34 antibody with FITCImage from Lydon H et al., BMC veterinary research., 14 (1) 47. Fig 5.; doi: 10.1186/s12917-018-1332-4. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/.

    Lydon et al. used ab188285 FITC Conjugation Kit to label mouse anti-sheep CD34 antibody with FITC.

    Data shows a) Representative light-scatter (SSC-A) vs fluorescence (CD34-FITC) plot of CD34 expression on cells from ovine apheresis samples using two-colour flow cytometry. B) IgG control.

  • FITC Conjugation Kit labeling anti MUC1 SP antibodies FACS
    FITC Conjugation Kit labeling anti MUC1 SP antibodies FACSImage from Kovjazin R et al., PLoS One, 9(1):e85400. Fig 3.; doi: 10.1371/journal.pone.0085400. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

References (10)

Publishing research using ab188285? Please let us know so that we can cite the reference in this datasheet.

ab188285 has been referenced in 10 publications.

  • Charras A  et al. JAK Inhibitors Suppress Innate Epigenetic Reprogramming: a Promise for Patients with Sjögren's Syndrome. Clin Rev Allergy Immunol 58:182-193 (2020). PubMed: 31165348
  • Zhdanov DD  et al. Murine regulatory T cells induce death of effector T, B, and NK lymphocytes through a contact-independent mechanism involving telomerase suppression and telomere-associated senescence. Cell Immunol 331:146-160 (2018). PubMed: 29935763
  • Lydon H  et al. Peripheral mononuclear blood cell apheresis in a preclinical ovine model. BMC Vet Res 14:47 (2018). PubMed: 29439735
  • Wülfing C  et al. Neural architecture in lymphoid organs: Hard-wired antigen presenting cells and neurite networks in antigen entrance areas. Immun Inflamm Dis 6:354-370 (2018). PubMed: 29635889
  • Junginger J  et al. Zoonotic intestinal helminths interact with the canine immune system by modulating T cell responses and preventing dendritic cell maturation. Sci Rep 7:10310 (2017). PubMed: 28871165
  • Ma L  et al. SjCRT, a recombinant Schistosoma japonicum calreticulin, induces maturation of dendritic cells and a Th1-polarized immune response in mice. Parasit Vectors 10:570 (2017). PubMed: 29132406
  • Saresella M  et al. The NLRP3 and NLRP1 inflammasomes are activated in Alzheimer's disease. Mol Neurodegener 11:23 (2016). PubMed: 26939933
  • Wülfing C & Günther HS Dendritic cells and macrophages neurally hard-wired in the lymph node. Sci Rep 5:16866 (2015). PubMed: 26581550
  • Jiang D  et al. Alteration in 5-hydroxymethylcytosine-mediated epigenetic regulation leads to Purkinje cell vulnerability in ATM deficiency. Brain 138:3520-36 (2015). PubMed: 26510954
  • Pilakka-Kanthikeel S  et al. Sterile alpha motif and histidine/aspartic acid domain-containing protein 1 (SAMHD1)-facilitated HIV restriction in astrocytes is regulated by miRNA-181a. J Neuroinflammation 12:66 (2015). PubMed: 25890101

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