FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
- Datasheet
- References (7)
- Protocols
Overview
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Product name
FITC Conjugation Kit (Fast) - Lightning-Link®
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Product overview
FITC Conjugation Kit (Fast) (ab188285) uses a simple and quick process to conjugate an antibody to FITC / Fluorescein. It can also be used to conjugate other proteins or peptides.
This product is manufactured by Expedeon, an Abcam company. See the table below to match Abcam kit size against Expedeon product code.
To conjugate an antibody to FITC / Fluorescein using this kit:
- add modifier to antibody and incubate for 15 mins
- add quencher and incubate for 5 mins
The conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to FITC.
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Notes
Amount and volume of antibody for conjugation to FITC
Kit size Expedeon product code Recommended
amount of antibody1Maximum
amount of antibodyMaximum antibody
volume230 µg 310-0030 3 x 10 µg 3 x 20 µg 3 x 10 µL 100 µg 310-0005 1 x 100 µg 1 x 200 µg 1 x 100µL 300 µg 310-0010 3 x 100 µg 3 x 200 µg 3 x 100 µL 1 mg 310-0015 1 mg 2 mg 1 mL 1 Using the maximum amount of antibody may result in less labelling per antibody.
2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 5mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.50mM / 0.6% Tris1 0.1% BSA2 50% glycerol 0.1% sodium azide PBS Potassium phosphate Sodium chloride HEPES Sucrose Sodium citrate EDTA Trehalose 1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.Incompatible buffer constituents
Thiomerosal Proclin Glycine Arginine Glutathione DTT If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
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Tested applications
Suitable for: Conjugationmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 300 µg 1 mg 30 µg 100 µg Fluorescein Conjugate 3 vials 1 vial 3 vials 1 vial Fluorescein Modifier reagent 1 vial 1 vial 1 vial 1 vial Fluorescein Quencher reagent 1 vial 1 vial 1 vial 1 vial -
Research areas
Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab188285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Conjugation | Use at an assay dependent concentration. |
Images
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ab188285 FITC Conjugation Kit used to label mouse anti-sheep CD34 antibody with FITCImage from Lydon H et al., BMC veterinary research., 14 (1) 47. Fig 5.; doi: 10.1186/s12917-018-1332-4. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/.Lydon et al. used ab188285 FITC Conjugation Kit to label mouse anti-sheep CD34 antibody with FITC.
Data shows a) Representative light-scatter (SSC-A) vs fluorescence (CD34-FITC) plot of CD34 expression on cells from ovine apheresis samples using two-colour flow cytometry. B) IgG control.
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conjugation
Protocols
References
This product has been referenced in:
- Zhdanov DD et al. Murine regulatory T cells induce death of effector T, B, and NK lymphocytes through a contact-independent mechanism involving telomerase suppression and telomere-associated senescence. Cell Immunol 331:146-160 (2018). Read more (PubMed: 29935763) »
- Lydon H et al. Peripheral mononuclear blood cell apheresis in a preclinical ovine model. BMC Vet Res 14:47 (2018). Read more (PubMed: 29439735) »
