Product nameFITC Conjugation Kit - Lightning-Link®
See all FITC kits
This product is manufactured by Expedeon, an Abcam company. Expedeon product code 707-0010 was previously called Lightning-Link® Fluorescein Labeling Kit 3 reactions each up to 200 µg and is the same as the 3 x 100 µg size of this product. Expedeon product code 707-0030 was previously called Lightning-Link® Fluorescein Labeling Kit 3 reactions each up to 20 µg and is the same as the 3 x 10 µg size of this product. Expedeon product code 707-0015 was previously called Lightning-Link® Fluorescein Labeling Kit 1 reaction up to 2 mg and is the same as the 1 mg size of this product. Expedeon product code 707-0005 was previously called Lightning-Link® Fluorescein Labeling Kit 1 reaction up to 200 µg and is the same as the 100 µg size of this product.
FITC Conjugation Kit / FITC Labeling Kit ab102884 uses a simple and quick process for FITC labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.
We recommend FITC Conjugation Kit ab188285 as an alternative this kit using a faster, newer protocol.
To conjugate an antibody to FITC using this kit:
- add modifier to antibody and incubate for 3 hrs
- add quencher and incubate for 30 mins
The FITC conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to FITC.
Amount and volume of antibody for conjugation to FITC
amount of antibody1
amount of antibody
3 x 10 µg
3 x 20 µg
3 x 10 µL
1 x 100 µg
1 x 200 µg
1 x 100 µL
3 x 100 µg
3 x 200 µg
3 x 100 µL
1 Using the maximum amount of antibody may result in less labelling per antibody.
2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 5mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
50mM / 0.6% Tris1 0.1% BSA 50% glycerol 0.1% sodium azide PBS Potassium phosphate Sodium chloride HEPES Sucrose Sodium citrate EDTA Trehalose
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
Incompatible buffer constituents
Thiomerosal Proclin Glycine Arginine Glutathione DTT
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture.
Tested applicationsSuitable for: Conjugationmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 mg 100 µg 3 x 10 µg 3 x 100 µg 300 µg 30 µg FITC mix 1 vial 1 vial 3 vials 3 x 100µg 3 x 100µg 3 vials Modifier reagent 1 vial 1 vial 1 vial 1 vial 1 vial 1 vial Quencher reagent 1 vial 1 vial 1 vial 1 vial 1 vial 1 vial
Our Abpromise guarantee covers the use of ab102884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Conjugation||Use at an assay dependent dilution.|
This product has been referenced in:
- Li Q et al. Megalin mediates plasma membrane to mitochondria cross-talk and regulates mitochondrial metabolism. Cell Mol Life Sci 75:4021-4040 (2018). Read more (PubMed: 29916093) »
- Zhdanov DD et al. Murine regulatory T cells induce death of effector T, B, and NK lymphocytes through a contact-independent mechanism involving telomerase suppression and telomere-associated senescence. Cell Immunol 331:146-160 (2018). Read more (PubMed: 29935763) »