For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
How much FITC (moles, ideally) is in each vial of the 3 x 100-200ug conjugation kit? We hope to achieve a ratio of 2 FITC molecules perIgG molecule. Is there any data for a reference antibody, demonstrating the number of FITC molecules conjugated per IgG, for a given starting concentration of IgG (e.g., 1mg/ml)and a given formulation (e.g., PBS, azide-free, carrier--free)?
Asked on Sep 24 2012
Thank you for your interest in the FITC conjugation kit. Unfortunately, it will be difficult to achieve a consistent ratio of FITC : IgG without extensive testing of the kit to determine the appropriate conditions. We have not done this testing, as the kit was developed for ease of producing a conjugate that simply gives a bright signal, rather than producing a conjugate of a known ratio. Further, the components of the reagents are considered proprietary, which makes knowing where to start difficult. To do what you want, I think it will make more sense to work with a standard FITC conjugation protocol, using known reagents and quantities, and testing various combination of FITC and antibody.
Answered on Sep 24 2012