For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Inquiry: In labeling an antibody with your Easylink, I would like to know to which residue you are attaching the reagent. How do you know it isn't attaching to the FAB portion?
Asked on Dec 19 2012
Thank you for contacting us.
The labeling kit works by targeting free amines groups on your antibody. We therefore cannot guarantee that the label will not bind to the FAB area, however this would be the case for any other amine orientated labeling technology. Although this might seem like an issue , it does not mean that the FAB will become unreactive, and we see successful conjugations in the great majority of cases.
The amine binding would only become an issue if there is an amine group within the antibody binding site itself.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447
Answered on Dec 19 2012