Key features and details
- FITC Rat monoclonal [25B6] to Ctip2
- Suitable for: ICC, Flow Cyt
- Reacts with: Mouse, Human, Zebrafish
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Product nameFITC Anti-Ctip2 antibody [25B6]
See all Ctip2 primary antibodies
DescriptionFITC Rat monoclonal [25B6] to Ctip2
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: ICC, Flow Cytmore details
Species reactivityReacts with: Mouse, Human, Zebrafish
Recombinant fragment corresponding to Human Ctip2 aa 1-150.
- Jurkat cells.
Product was previously marketed under the MitoSciences sub-brand.
Fluorochrome/Protein Ratio: at least 3 moles FITC per mole of IgG.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurity is near homogeneity as judged by SDS-PAGE. ab123449 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.
Our Abpromise guarantee covers the use of ab123449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 0.125 - 1 µg/ml.|
|Flow Cyt||Use a concentration of 0.125 - 1 µg/ml.|
FunctionTumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
Tissue specificityHighly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
Sequence similaritiesContains 6 C2H2-type zinc fingers.
- Information by UniProt
- ATL1 alpha antibody
- ATL1 antibody
- ATL1 beta antibody
ab123449 at 0.125µg/ml staining Ctip2 in Jurkat cells by Flow Cytometry (red) or an equal amount of an FITC conjugated control antibody (black) in 3% BSA block.The Jurkat cells show a clear CTIP2 specific signal
ab123449 at 0.125µg/ml staining CTIP2 in Jurkat cells by Immunocytochemistry. (N.B. cells were stained for 2h at room temperature in 3% BSA blocking solution.)
ab123449 at 0.125µg/ml staining Ctip2 in SH-SY5Y cells by Flow Cytometry (red) or an equal amount of an FITC conjugated control antibody (black) in 3% BSA block. The SH-SY5Y cells do not show significant CTIP2 signal over the negative control signal.
ab123449 has been referenced in 2 publications.
- Del Toro D et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons. Cell 180:323-339.e19 (2020). PubMed: 31928845
- Forouzanfar F et al. HIV-1 Vpr mediates the depletion of the cellular repressor CTIP2 to counteract viral gene silencing. Sci Rep 9:13154 (2019). PubMed: 31511615