FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)

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Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)
  • Immunocytochemistry - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)
  • Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)

Key features and details

  • FITC Mouse monoclonal [C-04] to Cytokeratin 18
  • Suitable for: ICC, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

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Overview

  • Product name

    FITC Anti-Cytokeratin 18 antibody [C-04]
    See all Cytokeratin 18 primary antibodies

  • Description

    FITC Mouse monoclonal [C-04] to Cytokeratin 18

  • Host species

    Mouse

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: ICC, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mammals

  • Immunogen

    Tissue, cells or virus corresponding to Human Cytokeratin 18. Cytoskeleton preparation of epidermal carcinoma cell line A431
    Database link: P05783

  • Positive control

    • ICC: HCT116 cells; Flow Cyt (Intra): HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use a concentration of 5 µg/ml.
Flow Cyt (Intra)
Use a concentration of 1 - 2 µg/ml.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
ICC
Use a concentration of 5 µg/ml.
Flow Cyt (Intra)
Use a concentration of 1 - 2 µg/ml.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

  • Tissue specificity

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

  • Involvement in disease

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Post-translational
    modifications

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.

  • Cellular localization

    Cytoplasm > perinuclear region.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cell proliferation inducing gene 46 protein antibody
      • Cell proliferation inducing protein 46 antibody
      • Cell proliferation-inducing gene 46 protein antibody
      • CK 18 antibody
      • CK-18 antibody
      • CK18 antibody
      • CYK 18 antibody
      • CYK18 antibody
      • Cytokeratin 18 antibody
      • Cytokeratin endo B antibody
      • Cytokeratin-18 antibody
      • K 18 antibody
      • K18 antibody
      • K1C18_HUMAN antibody
      • KA18 antibody
      • Keratin 18 antibody
      • Keratin 18, type I antibody
      • Keratin D antibody
      • keratin, type I cytoskeletal 18 antibody
      • Keratin-18 antibody
      • Krt18 antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)
      Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)

      Overlay histogram showing intracellular staining of HeLa cells labeled with ab52459 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52459, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (FITC) (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunocytochemistry - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)
      Immunocytochemistry - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)
      ICC/IF image of ab52459 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52459, 5µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
    • Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)
      Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)

      Flow cytometry (Intracellular) analysis HeLa (human cervix carcinoma cell line) cells labeling Cytokeratin 18 with ab52459 (red). Overlay with Isotype mouse IgG1 control FITC antibody (blue).

    References (8)

    Publishing research using ab52459? Please let us know so that we can cite the reference in this datasheet.

    ab52459 has been referenced in 8 publications.

    • Chen KL  et al. SIRT7 Regulates Lipopolysaccharide-Induced Inflammatory Injury by Suppressing the NF-?B Signaling Pathway. Oxid Med Cell Longev 2019:3187972 (2019). PubMed: 31285783
    • Crowell PD  et al. Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate. Cell Rep 28:1499-1510.e6 (2019). PubMed: 31390564
    • Chen J  et al. The Potential of the FSP1cre-Pparb/d-/- Mouse Model for Studying Juvenile NAFLD. Int J Mol Sci 20:N/A (2019). PubMed: 31618976
    • Hu B  et al. Induction of Chemerin on Autophagy and Apoptosis in Dairy Cow Mammary Epithelial Cells. Animals (Basel) 9:N/A (2019). PubMed: 31640289
    • Zhou YF  et al. Methionine and choline supply alter transmethylation, transsulfuration, and cytidine 5'-diphosphocholine pathways to different extents in isolated primary liver cells from dairy cows. J Dairy Sci 101:11384-11395 (2018). PubMed: 30316602
    • Liew PX  et al. iNKT Cells Orchestrate a Switch from Inflammation to Resolution of Sterile Liver Injury. Immunity 47:752-765.e5 (2017). PubMed: 29045904
    • Rando G  et al. Glucocorticoid receptor-PPARa axis in fetal mouse liver prepares neonates for milk lipid catabolism. Elife 5:N/A (2016). PubMed: 27367842
    • Chauchereau A  et al. Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer. Exp Cell Res 317:262-75 (2011). Flow Cyt ; Human . PubMed: 20974126

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