FITC Anti-Cytomegalovirus AD169 antibody (ab30952)

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Key features and details

  • FITC Goat polyclonal to Cytomegalovirus AD169
  • Suitable for: ICC, IHC-P
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

Overview

  • Product name

    FITC Anti-Cytomegalovirus AD169 antibody
    See all Cytomegalovirus AD169 primary antibodies

  • Description

    FITC Goat polyclonal to Cytomegalovirus AD169

  • Host species

    Goat

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Specificity

    ab30952 recognises Cytomegalovirus strain AD169.

  • Tested applications

    Suitable for: ICC, IHC-Pmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Cytomegalovirus AD169

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purification notes

    Chromatographically purified IgG fraction.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab30952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance

    Cytomegalovirus (CMV) is a member of the Herpes virus family. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid which is embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope that is decorated with virus-specific glycoprotein spikes. The viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. Cytomegalovirus strain AD169 is a laboratory-adapted strain and appears to lack a 15kb region of the 200kb genome that is present in clinical isolates. This region contains 19 open reading frames whose functions have yet to be elucidated. AD169 is also unique in that it is unable to enter latency and nearly always assumes lytic growth upon infection.

  • Alternative names

    • HCMV antibody
    • HHV5 antibody
    • Human Herpesvirus 5 antibody
    • UL55 (strain AD169) antibody

References (0)

Publishing research using ab30952? Please let us know so that we can cite the reference in this datasheet.

ab30952 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Was this antibody raised against only intact virus or were internal virus particles present in the immunogen? I'd like to use this antibody to detect the internal viral protein, PP65 (also known as UL83).

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Answer

Thank you very much for your call earlier this week and for your patience while I have been in touch with the lab regarding your enquiry.



I've heard back from the lab, and the immunogen for thisantibody is purified virions of strain AD169. Theantibody recognizes predominantly surface proteins, in particular glycoprotein B (gB), so I think this antibody will not be suitable for detecting PP65.



If I remember correctly from our phone conversation, our PP65 antibodies were not suitable for your experimental design, but if you do have any questions about any of these antibodies or need anything else, please let me know and I'll be happy to help.

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