FITC Anti-DMPO Nitrone Adduct antibody [N1664A] (ab110429)

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Immunocytochemistry/ Immunofluorescence - FITC Anti-DMPO Nitrone Adduct antibody [N1664A] (ab110429)

    Key features and details

    • FITC Mouse monoclonal [N1664A] to DMPO Nitrone Adduct
    • Suitable for: ICC
    • Reacts with: Species independent
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    Overview

    • Product name

      FITC Anti-DMPO Nitrone Adduct antibody [N1664A]
      See all DMPO Nitrone Adduct primary antibodies

    • Description

      FITC Mouse monoclonal [N1664A] to DMPO Nitrone Adduct

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Specificity

      ab110429 recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA.

    • Tested applications

      Suitable for: ICCmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.

    • Positive control

      • ICC: Raw 264.7 cells.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab110429 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC 1/100.

    Target

    • Relevance

      For measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts.

    • Alternative names

      • 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody

    Images

    • Immunocytochemistry/ Immunofluorescence - FITC Anti-DMPO Nitrone Adduct antibody [N1664A] (ab110429)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-DMPO Nitrone Adduct antibody [N1664A] (ab110429)
      ab110429 staining DMPO Nitrone Adduct in Raw264.7 cells by Immunocytochemistry/ Immunofluorescence. Left panels represent cells treat with DMPO, while right panels represent those not treated. Cells were counterstained blue with DAPI. Protocol can be found here.

    References (1)

    Publishing research using ab110429? Please let us know so that we can cite the reference in this datasheet.

    ab110429 has been referenced in 1 publication.

    • Carmo LS  et al. Expansive Vascular Remodeling and Increased Vascular Calcification Response to Cholecalciferol in a Murine Model of Obesity and Insulin Resistance. Arterioscler Thromb Vasc Biol 39:200-211 (2019). PubMed: 30580565

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