Key features and details
- FITC Mouse monoclonal [15A3] to DNA/RNA Damage
- Suitable for: ICC/IF, IHC-P, IHC-Fr
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Product nameFITC Anti-DNA/RNA Damage antibody [15A3]
See all DNA/RNA Damage primary antibodies
DescriptionFITC Mouse monoclonal [15A3] to DNA/RNA Damage
ConjugationFITC. Ex: 493nm, Em: 528nm
Specificity15A3 binds with high specificity and affinity to oxo8dG (8-hydroxy-2'-deoxyguanosine), oxo8Gua (8-oxo-7,8-dihydroguanine), and oxo8G (8-oxo-7,8-dihydroguanosine) present in biological fluids with Kaff values of 9.2 x 108 M-1, 4.7 x' 17 M-1, and 2.1 x 108 M-1, respectively.
Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Frmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to DNA/RNA Damage. 8-hydroxy-guanosine-BSA and -casein conjugates.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab183393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 - 10 µg/ml.|
|IHC-Fr||Use a concentration of 1 - 10 µg/ml.|
RelevanceIn intact animals, lesions (adducts) excised from DNA are transported from the cell through the circulation and excreted in urine. In bacteria, DNA adducts are excreted directly into the medium. In either case, the adducts can be assayed as a measure of oxidative damage to DNA. In particular, Oxo-8-dG (8-Oxo-7,8-dihydro-2'-deoxyguanosine) serves as an excellent marker for DNA damage produced by oxidants because it represents one of the major products generated by a wide array of treatments associated with oxidant damage such as that produced by irradiation and various carcinogens and because it is implicated in spontaneous transversion mutagenesis. Oxo-8-Gua (8-oxo-7,8-dihydroguanine) is one of the most common DNA lesions resulting from reactive oxygen species and can result in a mismatched pairing with adenine resulting in G to T and C to A substitutions in the genome. In humans, it is primarily repaired by DNA glycosylase OGG1. It can be caused by ionizing radiation, in connection with oxidative metabolism. Oxo-8-G (8-oxo-7,8-dihydroguanosine) is classified as an oxidized ribonucleotide, and is primarily used in studies of oxidative RNA damage and associated RNA repair and RNA turnover mechanisms within the cell. In the cell, Oxo-8-G RNA lesions are formed by reaction with reactive oxygen species (ROS) generated either via normal oxidative metabolic processes, UV ionizing radiation, or exposure to oxidative agents. Oxidative RNA damage can lead to defects in protein synthesis, for example, decreased rates of protein synthesis and production of aggregated or truncated peptides, with important implications in aging and neurodegenerative disorders and artherosclerosis.
- 7,8-Dihydro-8-oxo-2'-deoxyguanosine antibody
- 7,8-Dihydro-8-oxodeoxyguanosine antibody
- 7,8-Dihydro-8-oxoguanine antibody
ab183393 has been referenced in 4 publications.
- Rodius S et al. Fisetin protects against cardiac cell death through reduction of ROS production and caspases activity. Sci Rep 10:2896 (2020). PubMed: 32076073
- Kurita H et al. Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation. PLoS One 15:e0232724 (2020). PubMed: 32374749
- Tempka D et al. Downregulation of PARP1 transcription by CDK4/6 inhibitors sensitizes human lung cancer cells to anticancer drug-induced death by impairing OGG1-dependent base excision repair. Redox Biol 15:316-326 (2018). PubMed: 29306194
- Park EM et al. Assay of excised oxidative DNA lesions: isolation of 8-oxoguanine and its nucleoside derivatives from biological fluids with a monoclonal antibody column. Proc Natl Acad Sci U S A 89:3375-9 (1992). PubMed: 1565629