FITC Anti-DR5 antibody [DR5-01-1] (ab53319)
![Flow Cytometry - FITC Anti-DR5 antibody [DR5-01-1] (ab53319)](https://www.abcam.com/ps/products/53/ab53319/Images/ab53319-454768-FITC-AntiDR5-antibody-Flow.jpg "Flow Cytometry - FITC Anti-DR5 antibody [DR5-01-1] (ab53319)")
Key features and details
- FITC Mouse monoclonal [DR5-01-1] to DR5
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
Overview
-
Product name
FITC Anti-DR5 antibody [DR5-01-1]
See all DR5 primary antibodies -
Description
FITC Mouse monoclonal [DR5-01-1] to DR5 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fusion protein of human IgG heavy chain and extracellular domain of DR5.
-
General notes
The reagent is free of unconjugated FITC.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
DR5-01-1 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
|
| Notes |
|---|
|
Flow Cyt
Use a concentration of 1 - 5 µg/ml. |
Target
-
Function
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. -
Tissue specificity
Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. -
Involvement in disease
Squamous cell carcinoma of the head and neck -
Sequence similarities
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 8795 Human
- Omim: 603612 Human
- SwissProt: O14763 Human
- Unigene: 521456 Human
- Unigene: 661668 Human
-
Alternative names
- Fas like protein antibody
- Apoptosis inducing protein TRICK2A/2B antibody
- Apoptosis inducing receptor TRAIL R2 antibody
see all
Images
-
Flow Cytometry - FITC Anti-DR5 antibody [DR5-01-1] (ab53319)
Flow cytometry analysis (surface staining) of CD262 on CD262-transfectants using ab53319 at 5 μg/ml.
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab53319 has been referenced in 2 publications.
- Strekalova E et al. Preclinical Breast Cancer Models to Investigate Metabolic Priming by Methionine Restriction. Methods Mol Biol 1866:61-73 (2019). PubMed: 30725408
- Pan L et al. Higher-Order Clustering of the Transmembrane Anchor of DR5 Drives Signaling. Cell 176:1477-1489.e14 (2019). PubMed: 30827683