FITC Anti-E. coli O + E. coli K antibody (ab20856)

Submit a review Q&A (4)References (1)

Key features and details

  • FITC Goat polyclonal to E. coli O + E. coli K
  • Suitable for: ICC/IF
  • Reacts with: Escherichia coli
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

Overview

  • Product name

    FITC Anti-E. coli O + E. coli K antibody
    See all E. coli O + E. coli K primary antibodies

  • Description

    FITC Goat polyclonal to E. coli O + E. coli K

  • Host species

    Goat

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Escherichia coli

  • Immunogen

    Tissue, cells or virus corresponding to Escherichia coli E. coli O + E. coli K. Mixture of E. coli serotypes (E. coli).

  • General notes

    The antibody is covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Associated products

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Notes
Application notes
IF: Use at an assay dependent dilution.
Acetone fixation of the antigen source is recommended prior to staining. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

  • Relevance

    Escherichia coli (E. coli) are Gram negative enteric bacteria that live in the human gut. Its presence in nature is an indication of human feacal pollution. E. coli also causes human disease such as urinary tract infections and neonatal meningitis. Infection with particular enterotoxigenic strains can lead to life threatening intestinal diseases. E. coli are distinguished immunologically by serotyping based on three types of antigen: the somatic or O antigen which corresponds to terminal sugars on the cell surface lipopolysaccharide, the capsular or K antigens and the flagellar or H antigens.

  • Alternative names

    • Capsular antigen antibody
    • Escherichia coli antibody
    • Escherichia coli K antibody
    • Escherichia coli O antibody
    • K antigen antibody
    • O antigen antibody
    see all

References (1)

Publishing research using ab20856? Please let us know so that we can cite the reference in this datasheet.

ab20856 has been referenced in 1 publication.

  • Zhao WD  et al. Caspr1 is a host receptor for meningitis-causing Escherichia coli. Nat Commun 9:2296 (2018). PubMed: 29895952

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

Thank you for your response! Very helpful.

I think I’ll have to test for enterobacteriaceae cross-reactivity myself.

Best regards

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Answer

I'm glad you found the information of help.

If you do test any of these antibodies we would be very interested to hear of the results, regardless of if they are positive or not. We like to encourage all of our customers to submit an Abreview via the online product datasheet with any data they collect with our antibodies. As a reward for doing this, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

If you let me know which antibody in particular you are interested in using I may be able to provide you with a testing discount code. More information on this scheme can be found form the following link:

www.abcam.com/collaborationdiscount

I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

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Question

Phone call requesting information on if any of the anti-E. coli antibodies have been tested for their specificity.

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Answer

Thank you for contacting us and your interest in our products.

I can now share some more information relating to the antibodies we have directed towards E. coli (O and K). We have two different antibodies directed against this bacterium, with different conjugation available:

ab31499, unlabelled(ab33604, FITC; ab33837, HRP; ab33839, Biotin conjugated of same antibody preparation as ab31499)

The immunogen for these antibodies is a mixture of the following E. coli strains: O157:H7, O20, O125, O55, O111 & K12. The mixture of these strains was of intact plus lysed bacteria which had not been denatured in any way. All E. coli have many surface and internal antigens in common and this antibody recognizes this large mixture and therefore reacts with most, if not all, E. coli. Theseantibodies have been tested against the strains listed above but have not been cross-absorbed and may react with related Enterobacteriaceae. These potential cross-reacting strains have not been identified.

ab20765, ab20856 and ab21110 are Biotin, FITC and HRP conjugates of the same antibody.

These antibodies have been raised against a mixture of E. coli serotypes.These antibodies have been tested for reactivity against the immunogen strains of E.coli but again they have not been absorbed and will most likely cross react withother related Enterobacteriaceae.



I am sorry that in this case I was not able to be of more help. If you have any further questions, please do not hesitate to contact us again.

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Question

Is the FITC conjugated to an amine or a carboxyl group on this antibody?

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Answer

Thank you for your enquiry. Good news: In ab20856, the FITC is conjugated to the amine group. Please let me know if you need anything further.

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Question

I noticed this datasheet says that the antibody reacts with many serotypes. Does this antibody react with K99 and O157?

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Answer

Thank you for your enquiry. Ab20856 is reactive with E. coli O157 but has not been tested with K99. I hope this information helps. Please do not hesitate to contact us again if you need anything further.

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