FITC Anti-E. coli O157 antibody (ab31550)

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Key features and details

  • FITC Goat polyclonal to E. coli O157
  • Suitable for: ELISA, ICC/IF
  • Reacts with: Escherichia coli
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

Overview

  • Product name

    FITC Anti-E. coli O157 antibody
    See all E. coli O157 primary antibodies

  • Description

    FITC Goat polyclonal to E. coli O157

  • Host species

    Goat

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: ELISA, ICC/IFmore details

  • Species reactivity

    Reacts with: Escherichia coli

  • Immunogen

    Tissue, cells or virus corresponding to Escherichia coli E. coli O157.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituents: PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    ab31550 is an affinity purified IgG.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Associated products

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab31550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/20 - 1/2000.
ICC/IF
Use at an assay dependent dilution.
Notes
ELISA
1/20 - 1/2000.
ICC/IF
Use at an assay dependent dilution.

Target

  • Relevance

    Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.

  • Alternative names

    • Escherichia coli O157 antibody

References (1)

Publishing research using ab31550? Please let us know so that we can cite the reference in this datasheet.

ab31550 has been referenced in 1 publication.

  • Battle SE  et al. Actin pedestal formation by enterohemorrhagic Escherichia coli enhances bacterial host cell attachment and concomitant type III translocation. Infect Immun 82:3713-22 (2014). ICC/IF . PubMed: 24958711

Customer reviews and Q&As

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Question

Dear Mr/Mrs,



I am using your antibody for specific detection of E. coli in gut samples. I get a fluorescent staining on positive and negative tissue’s and I cannot define the difference between background color or the real colouring of the bacteria. Is it possible that the antibody also recognizes VTEC (not only EHEC)?

Can you tell me to which part of the bacteria the antibody is bounded?



Thank you in advance

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Answer

Thank you for your enquiry regarding ab31550. I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

Please could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

Thank you for your understanding and co-operation in this matter.

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