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    fitc-epcam-antibody-b291-vu-1d9-ab8666.pdf

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FITC Anti-EpCAM antibody [B29.1 (VU-1D9)] (ab8666)

  • Datasheet
  • SDS
Reviews (2)Q&A (3)References (15)

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Immunocytochemistry/ Immunofluorescence - FITC Anti-EpCAM antibody [B29.1 (VU-1D9)] (ab8666)

    Key features and details

    • FITC Mouse monoclonal [B29.1 (VU-1D9)] to EpCAM
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant human EpCAM protein (ab155637)

    View more associated products

    Overview

    • Product name

      FITC Anti-EpCAM antibody [B29.1 (VU-1D9)]
      See all EpCAM primary antibodies
    • Description

      FITC Mouse monoclonal [B29.1 (VU-1D9)] to EpCAM
    • Host species

      Mouse
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Specificity

      This antibody reacts with a 40 kD cell surface glycoprotein called ESA.
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue, cells or virus corresponding to Human EpCAM. Raised against carcinoma cell line of human origin.
      Database link: P16422

    • Positive control

      • Breast, colon carcinoma and tonsil
    • General notes

      Dilute according to the particular application being used. In general, 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.

      Fluorescein (FITC) conjugated at a ratio of 6 moles of Fluorescein to 1 mole of antibody. Maximum excitation of FITC occurs at wavelength 492 nm and maximum emission occurs at wavelength 520 nm.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Please see notes section.
    • Storage buffer

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituent: 0.01% PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      B29.1 (VU-1D9)
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Tags & Cell Markers
      • Cell Type Markers
      • Epi / Endo-thelial

    Associated products

    • Alternative Versions

      • Alexa Fluor® 647 Anti-EpCAM antibody [VU-1D9] (ab239273)
    • Isotype control

      • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
    • Recombinant Protein

      • Recombinant human EpCAM protein (ab155637)

    Applications

    Our Abpromise guarantee covers the use of ab8666 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF Use a concentration of 5 - 10 µg/ml. See Abreview.

    Target

    • Function

      May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
    • Tissue specificity

      Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
    • Involvement in disease

      Defects in EPCAM are the cause of diarrhea type 5 (DIAR5) [MIM:613217]. It is an intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum.
      Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM.
    • Sequence similarities

      Belongs to the EPCAM family.
      Contains 1 thyroglobulin type-1 domain.
    • Post-translational
      modifications

      Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
    • Cellular localization

      Lateral cell membrane. Cell junction > tight junction. Co-localizes with CLDN7 at the lateral cell membrane and tight junction.
    • Target information above from: UniProt accession P16422 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4072 Human
      • Omim: 185535 Human
      • SwissProt: P16422 Human
      • Unigene: 542050 Human
      • Alternative names

        • 17 1A antibody
        • 323/A3 antibody
        • Adenocarcinoma associated antigen antibody
        • Adenocarcinoma-associated antigen antibody
        • Antigen identified by monoclonal antibody AUA1 antibody
        • AUA1 antibody
        • CD326 antibody
        • CD326 antigen antibody
        • Cell surface glycoprotein Trop 1 antibody
        • Cell surface glycoprotein Trop 2 antibody
        • Cell surface glycoprotein Trop-1 antibody
        • CO 17A antibody
        • CO17 1A antibody
        • CO17A antibody
        • DIAR5 antibody
        • EGP 2 antibody
        • EGP antibody
        • EGP2 antibody
        • EGP314 antibody
        • EGP40 antibody
        • Ep CAM antibody
        • Ep-CAM antibody
        • EPCAM antibody
        • EPCAM_HUMAN antibody
        • EpCAM1 antibody
        • Epithelial cell adhesion molecule antibody
        • Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) antibody
        • Epithelial cell surface antigen antibody
        • Epithelial cellular adhesion molecule antibody
        • Epithelial glycoprotein 1 antibody
        • Epithelial glycoprotein 314 antibody
        • Epithelial glycoprotein antibody
        • ESA antibody
        • GA733 1 antibody
        • GA733 2 antibody
        • GA733-2 antibody
        • gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein antibody
        • gp4 antibody
        • hEGP 2 antibody
        • hEGP314 antibody
        • HNPCC8 antibody
        • Human epithelial glycoprotein 2 antibody
        • KS 1/4 antigen antibody
        • KS1/4 antibody
        • KSA antibody
        • Ly74 antibody
        • Lymphocyte antigen 74 antibody
        • M1S 1 antibody
        • M1S2 antibody
        • M4S1 antibody
        • Major gastrointestinal tumor associated protein GA733 2 antibody
        • Major gastrointestinal tumor-associated protein GA733-2 antibody
        • mEGP314 antibody
        • Membrane component chromosome 4 surface marker (35kD glycoprotein) antibody
        • Membrane component, chromosome 4, surface marker 1 antibody
        • Membrane component, chromosome 4, surface marker antibody
        • MIC18 antibody
        • MK 1 antibody
        • Protein 289A antibody
        • TACD1 antibody
        • TACSTD1 antibody
        • TROP1 antibody
        • Tumor associated calcium signal transducer 1 antibody
        • Tumor associated calcium signal transducer 2 precursor antibody
        • Tumor-associated calcium signal transducer 1 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - FITC Anti-EpCAM antibody [B29.1 (VU-1D9)] (ab8666)
        Immunocytochemistry/ Immunofluorescence - FITC Anti-EpCAM antibody [B29.1 (VU-1D9)] (ab8666)
        ICC/IF image of ab8666 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8666, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      Protocols

      • Immunoprecipitation protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (15)

      Publishing research using ab8666? Please let us know so that we can cite the reference in this datasheet.

      ab8666 has been referenced in 15 publications.

      • Han T  et al. miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance. Mol Ther Nucleic Acids 19:1073-1085 (2020). PubMed: 32044726
      • Cheng Z  et al. Long non-coding RNA THOR promotes liver cancer stem cells expansion via ß-catenin pathway. Gene 684:95-103 (2019). PubMed: 30359743
      • Ran RZ  et al. miR-194 inhibits liver cancer stem cell expansion by regulating RAC1 pathway. Exp Cell Res 378:66-75 (2019). PubMed: 30844391
      • Jiang ZB  et al. miR-365 regulates liver cancer stem cells via RAC1 pathway. Mol Carcinog 58:55-65 (2019). PubMed: 30182377
      • Zhang P  et al. Dissecting the Single-Cell Transcriptome Network Underlying Gastric Premalignant Lesions and Early Gastric Cancer. Cell Rep 27:1934-1947.e5 (2019). PubMed: 31067475
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-5 of 5 Abreviews or Q&A

      Question

      Dear technical team,

      Our customer is looking for EpCAM antibody for human cell staining in mouse tissue so,
      she needs antibody does not react with mouse.
      She will test IF and IHC-P or IHC-Fr so I searched ab20160, ab89992, ab8666.
      Please let me know which one is right for our customer.

      Best regards

      Read More

      Abcam community

      Verified customer

      Asked on Aug 01 2012

      Answer

      Thank you for contacting us.

      As ab20160, ab89992, ab8666 have as yet not been tested with mouse samples, I would therefore not be able to say if they would cross react with the mouse tissue or not. I think a different antibody which may be more worthwhile trying is ab124825. This has been tested in mouse and found not to react, and as it is a monolconal, this reactivity is more likely to be maintained through different lots.

      This antibody has only been tested in WB, IHC-P andICC. If your customer would be interested in participating in the testing discount scheme to use the antibody in IHC-Fr and IF then I would be able to generate a discount code for them. If they would be interested in this I would need their email address and the institutethey are from.

      More information on the testing discount scheme can be found from the following link:

      https://www.abcam.com/collaborationdiscount

      I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

      Read More

      Abcam Scientific Support

      Answered on Aug 01 2012

      Question

      I was wondering if you could shed some light on the difference between this antibody and it's PE equivalent. The PE tagged version of this antibody does not have the B29.1 before the VU-ID9 tag, and I have had good success with the former. Would there be a difference in the two?

      Read More

      Abcam community

      Verified customer

      Asked on Feb 09 2012

      Answer

      Thank you for contacting us.

      These products are actually from different antibodies. Though they are both raised against EpCAM they have slightly different immunogen sequences and are different products. Each product is covered by our Abpromise guarantee. We guarantee that this product will work in applications and species that we have listed on the datasheet.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Feb 09 2012

      Flow Cytometry abreview for Anti-EpCAM antibody [B29.1 (VU-ID9)] (FITC)

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Human Cell (breast cancer cells)
      Specification
      breast cancer cells
      Permeabilization
      No
      Read More

      Dr. Enrique Barrajon

      Verified customer

      Submitted Dec 11 2006

      Immunocytochemistry/ Immunofluorescence abreview for Anti-EpCAM antibody [B29.1 (VU-ID9)] (FITC)

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (MCF7 breast cancer cells)
      Specification
      MCF7 breast cancer cells
      Read More

      Dr. Enrique Barrajon

      Verified customer

      Submitted Oct 31 2006

      Question

      Used in Flow Cytometry - datasheet use 1ug for 10tothe6 cells. He used 1ug to 10tothe 5 cells and got hardly any signal. Tried at 1:1 and got signal, but obviously used all ab at once for this. Mouse epithelium was tissue.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 04 2003

      Answer

      Thank you for your enquiry regarding Ab8666. We are very sorry to hear that you are having difficulty with this antibody. According to the datasheet, this antibody has been tested on human sample (breast, colon carcinoma and tonsil). The cross-reactivity with mouse protein has not been tested.

      Read More

      Abcam Scientific Support

      Answered on Nov 05 2003

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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