Key features and details
- FITC Rat monoclonal [BM8] to F4/80
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Product nameFITC Anti-F4/80 antibody [BM8]
See all F4/80 primary antibodies
DescriptionFITC Rat monoclonal [BM8] to F4/80
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse
BALB/c macrophages obtained from 14-day-old bone marrow cell cultures.
- Mouse macrophages and spleen cells.
This antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. It is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Store at +4°C. Store In the Dark.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride
Concentration information loading...
Primary antibody notesThis antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. It is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.
Our Abpromise guarantee covers the use of ab60343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/50 - 1/100.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionOrphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
Tissue specificityExpression is restricted to eosinophils.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain.
Cellular localizationCell membrane.
- Information by UniProt
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
ICC/IF image of ab60343 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60343, 1µg/ml, FITC conjugated) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Overlay histogram showing RAW 264.7 cells stained with ab60343 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60343, 1/100 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells) with secondary antibody DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a minimal signal in RAW 264.7 cells fixed with methanol (5 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
ab60343 has been referenced in 20 publications.
- Gu S et al. AKT3 deficiency in M2 macrophages impairs cutaneous wound healing by disrupting tissue remodeling. Aging (Albany NY) 12:6928-6946 (2020). PubMed: 32291381
- Xie X et al. Eliminating blood oncogenic exosomes into the small intestine with aptamer-functionalized nanoparticles. Nat Commun 10:5476 (2019). PubMed: 31792209
- Ford AJ et al. Fibroblasts stimulate macrophage migration in interconnected extracellular matrices through tunnel formation and fiber alignment. Biomaterials 209:88-102 (2019). PubMed: 31030083
- Shin SH et al. Fluorine MR Imaging Monitoring of Tumor Inflammation after High-Intensity Focused Ultrasound Ablation. Radiology 287:476-484 (2018). PubMed: 29369752
- Yang F et al. IRE1a aggravates ischemia reperfusion injury of fatty liver by regulating phenotypic transformation of kupffer cells. Free Radic Biol Med 124:395-407 (2018). PubMed: 29969718