FITC Anti-F4/80 antibody [BM8] (ab60343)

Submit a review Q&A (2)References (20)
Immunocytochemistry/ Immunofluorescence - FITC Anti-F4/80 antibody [BM8] (ab60343)
  • Flow Cytometry - FITC Anti-F4/80 antibody [BM8] (ab60343)

Key features and details

  • FITC Rat monoclonal [BM8] to F4/80
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG2a

Overview

  • Product name

    FITC Anti-F4/80 antibody [BM8]
    See all F4/80 primary antibodies

  • Description

    FITC Rat monoclonal [BM8] to F4/80

  • Host species

    Rat

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    BALB/c macrophages obtained from 14-day-old bone marrow cell cultures.

  • Positive control

    • Mouse macrophages and spleen cells.

  • General notes

    This antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. It is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at +4°C. Store In the Dark.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% Gelatin, 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride
  • Concentration information loading...

  • Purity

    Affinity purified

  • Primary antibody notes

    This antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. It is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.

  • Clonality

    Monoclonal

  • Clone number

    BM8

  • Isotype

    IgG2a

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab60343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/100.

ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

  • Tissue specificity

    Expression is restricted to eosinophils.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ADGRE1 antibody
      • Adhesion G protein coupled receptor E1 antibody
      • Adhesion G protein-coupled receptor E1 antibody
      • AGRE1_HUMAN antibody
      • Cell surface glycoprotein EMR1 antibody
      • Cell surface glycoprotein F4/80 antibody
      • DD7A5 7 antibody
      • Egf like module containing mucin like hormone receptor like 1 antibody
      • Egf like module containing mucin like hormone receptor like sequence 1 antibody
      • EGF like module receptor 1 antibody
      • EGF TM7 antibody
      • EGF-like module receptor 1 antibody
      • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
      • EGFTM7 antibody
      • EMR 1 antibody
      • EMR1 antibody
      • EMR1 hormone receptor antibody
      • Gpf480 antibody
      • Ly71 antibody
      • Lymphocyte antigen 71 antibody
      • TM7LN3 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - FITC Anti-F4/80 antibody [BM8] (ab60343)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-F4/80 antibody [BM8] (ab60343)

      ICC/IF image of ab60343 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60343, 1µg/ml, FITC conjugated) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Flow Cytometry - FITC Anti-F4/80 antibody [BM8] (ab60343)
      Flow Cytometry - FITC Anti-F4/80 antibody [BM8] (ab60343)
      Overlay histogram showing RAW 264.7 cells stained with ab60343 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60343, 1/100 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells) with secondary antibody DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a minimal signal in RAW 264.7 cells fixed with methanol (5 min) used under the same conditions.

      Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    References (20)

    Publishing research using ab60343? Please let us know so that we can cite the reference in this datasheet.

    ab60343 has been referenced in 20 publications.

    • Gu S  et al. AKT3 deficiency in M2 macrophages impairs cutaneous wound healing by disrupting tissue remodeling. Aging (Albany NY) 12:6928-6946 (2020). PubMed: 32291381
    • Xie X  et al. Eliminating blood oncogenic exosomes into the small intestine with aptamer-functionalized nanoparticles. Nat Commun 10:5476 (2019). PubMed: 31792209
    • Ford AJ  et al. Fibroblasts stimulate macrophage migration in interconnected extracellular matrices through tunnel formation and fiber alignment. Biomaterials 209:88-102 (2019). PubMed: 31030083
    • Shin SH  et al. Fluorine MR Imaging Monitoring of Tumor Inflammation after High-Intensity Focused Ultrasound Ablation. Radiology 287:476-484 (2018). PubMed: 29369752
    • Yang F  et al. IRE1a aggravates ischemia reperfusion injury of fatty liver by regulating phenotypic transformation of kupffer cells. Free Radic Biol Med 124:395-407 (2018). PubMed: 29969718
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Question

    Hello. I am hoping to buy a flow cytometry antibody that sees human F4/80 antigen on peripheral blood mononuclear cell in vitro derived macrophages. Will the antibody ab60343 or any other antibody available from your company see human F4/80 on this cell type?

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    Answer

    Thank you for contacting us.

    Antibody ab60343 is a well-characterized F4/80 clone, BM8. If the cells express F4/80, the antibody will detect it. For more references, see the datasheet for the unconjugated version, ab16911:

    Click here (or use the following: https://www.abcam.com/F480-antibody-BM8-ab16911.html).

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    I have Rat mAb to F4/80 (BM8) ab 60343-100.　I want to know more about my antibody. Lot number is 526242. Could you send me the expiry date for this product.
    I am planning to use this antibody for IHC-P mouse heart. I would love to know if there is special recommendation/protocol for its use in IHC-P.

    Read More
    Answer

    Thank you for your enquiry.

    We do not have expire dates for our products and you should be able to use the product without anydifficulty if kept at the recommend storage temperature for at least one year. Please rest assured that our "Abpromise" guarantees a free of charge replacement or refund should the product not work as expectedwithin 6 months of purchase. If you have purchased this product more than a year ago, I would suggest to still try and use it.

    Regarding IHC-P protocol, please refer to the datasheet application information.

    IHC-P: 1/50. (fixation in 10% neutral buffered formalin for 24 h; blocking with non-immunized goat serum; microwaved for 6 min in citrate buffer; splenic macrophages as positive control.)

    I have also attached our recommended IHC-P protocol link for your reference.

    https://www.abcam.com/index.html?pageconfig=resource&rid=11384

    I hope this information will beuseful and help resolve any uncertainties you may have over the expire date. If there isanything else thatI canhelpyou with, please do not hesitate to contact me.

    Have a nice day!

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