Key features and details
- FITC Mouse monoclonal [LOB 3/17] to Fas
- Suitable for: Flow Cyt
- Reacts with: Human, Monkey, Rhesus monkey
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameFITC Anti-Fas antibody [LOB 3/17]
See all Fas primary antibodies
DescriptionFITC Mouse monoclonal [LOB 3/17] to Fas
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human, Monkey, Rhesus monkey
Fusion protein corresponding to Human Fas.
- HUT78 T cell line.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Clone numberLOB 3/17
Our Abpromise guarantee covers the use of ab33227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/1 - 1/10.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionReceptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Tissue specificityIsoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Involvement in diseaseDefects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
Sequence similaritiesContains 1 death domain.
Contains 3 TNFR-Cys repeats.
DomainContains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- ALPS 1A antibody
- ALPS1A antibody
- APO 1 antibody
ab33227 has been referenced in 1 publication.
- Chen JY et al. TNF-alpha renders human peritoneal mesothelial cells sensitive to anti-Fas antibody-induced apoptosis. Nephrol Dial Transplant 18:1741-7 (2003). PubMed: 12937219