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Cell Biology Apoptosis Receptors Death Receptors
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FITC Anti-Fas antibody [LOB 3/17] (ab33227)

  • Datasheet
  • SDS
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Flow Cytometry - FITC Anti-Fas antibody [LOB 3/17] (ab33227)

    Key features and details

    • FITC Mouse monoclonal [LOB 3/17] to Fas
    • Suitable for: Flow Cyt
    • Reacts with: Human, Monkey
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

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    Overview

    • Product name

      FITC Anti-Fas antibody [LOB 3/17]
      See all Fas primary antibodies
    • Description

      FITC Mouse monoclonal [LOB 3/17] to Fas
    • Host species

      Mouse
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Tested Applications & Species

      Application Species
      Flow Cyt
      Human
      See all applications and species data
    • Immunogen

      Fusion protein corresponding to Human Fas.

    • Positive control

      • HUT78 T cell line.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.09% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      LOB 3/17
    • Myeloma

      NS1
    • Isotype

      IgG1
    • Research areas

      • Cell Biology
      • Apoptosis
      • Receptors
      • Death Receptors
      • Immunology
      • Cell Type Markers
      • CD
      • Cytokines
      • Immunology
      • Innate Immunity
      • Cytokines
      • TNF Superfamily

    Associated products

    • Isotype control

      • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
    • Recombinant Protein

      • Recombinant human Fas protein (ab155635)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab33227 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Human
    Application Abreviews Notes
    Flow Cyt
    1/1 - 1/10.

    Use 10µl of the suggested working dilution to label 106 cells in 100µl.

     

     

     

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    1/1 - 1/10.

    Use 10µl of the suggested working dilution to label 106 cells in 100µl.

     

     

     

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
    • Tissue specificity

      Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
    • Involvement in disease

      Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
    • Sequence similarities

      Contains 1 death domain.
      Contains 3 TNFR-Cys repeats.
    • Domain

      Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P25445 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 355 Human
      • Omim: 134637 Human
      • SwissProt: P25445 Human
      • Unigene: 244139 Human
      • Alternative names

        • ALPS 1A antibody
        • ALPS1A antibody
        • APO 1 antibody
        • Apo 1 antigen antibody
        • APO 1 cell surface antigen antibody
        • Apo-1 antigen antibody
        • APO1 antibody
        • Apo1 antigen antibody
        • APO1 cell surface antigen antibody
        • Apoptosis antigen 1 antibody
        • Apoptosis mediating surface antigen FAS antibody
        • Apoptosis-mediating surface antigen FAS antibody
        • APT 1 antibody
        • APT1 antibody
        • CD 95 antibody
        • CD 95 antigen antibody
        • CD95 antibody
        • CD95 antigen antibody
        • Delta Fas antibody
        • Delta Fas/APO 1/CD95 antibody
        • Delta Fas/APO1/CD95 antibody
        • Fas (TNF receptor superfamily, member 6) antibody
        • FAS 1 antibody
        • FAS 827dupA antibody
        • Fas AMA antibody
        • Fas antibody
        • FAS Antigen antibody
        • Fas cell surface death receptor antibody
        • FAS1 antibody
        • FASLG receptor antibody
        • FASTM antibody
        • sFAS antibody
        • Surface antigen APO1 antibody
        • TNF receptor superfamily, member 6 antibody
        • TNFRSF 6 antibody
        • TNFRSF6 antibody
        • TNR6_HUMAN antibody
        • Tumor necrosis factor receptor superfamily member 6 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-Fas antibody [LOB 3/17] (ab33227)
        Flow Cytometry - FITC Anti-Fas antibody [LOB 3/17] (ab33227)
        Ab33227, used neat or at a dilution of 1/10 (and then using 10µl of the suggested working dilution to label 106 cells in 100µl), staining CD95 in HUT78 T cell line by Flow Cytometry.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab33227? Please let us know so that we can cite the reference in this datasheet.

      ab33227 has been referenced in 1 publication.

      • Chen JY  et al. TNF-alpha renders human peritoneal mesothelial cells sensitive to anti-Fas antibody-induced apoptosis. Nephrol Dial Transplant 18:1741-7 (2003). PubMed: 12937219

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