FITC Anti-Fibrinogen antibody (ab73925)
Key features and details
- FITC Chicken polyclonal to Fibrinogen
- Reacts with: Rat, Rabbit, Human, Pig
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgY
Overview
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Product name
FITC Anti-Fibrinogen antibody
See all Fibrinogen primary antibodies -
Description
FITC Chicken polyclonal to Fibrinogen -
Host species
Chicken -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Species reactivity
Reacts with: Rat, Rabbit, Human, Pig -
Immunogen
Purified full length human Fibrinogen.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 0.87% Sodium chloride, 0.328% Sodium phosphate -
Concentration information loading... -
Purity
IgY fraction -
Purification notes
The IgY fraction is isolated by a two step PEG precipitation procedure followed by ammonium sulphate precipitation. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
Applications
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
Post-translational
modificationsThe alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracellular medium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2243 Human
- Entrez Gene: 2244 Human
- Entrez Gene: 2266 Human
- Entrez Gene: 100514354 Pig
- Entrez Gene: 403164 Pig
- Entrez Gene: 24366 Rat
- Entrez Gene: 24367 Rat
- Entrez Gene: 361969 Rat
see all -
Alternative names
- FGA antibody
- FGB antibody
- FGG antibody
see all
References (1)
ab73925 has been referenced in 1 publication.
- Verhaegen C et al. Early thrombogenicity of coronary stents: comparison of bioresorbable polymer sirolimus-eluting and bare metal stents in an aortic rat model. Am J Cardiovasc Dis 10:72-83 (2020). PubMed: 32685265
