RecombinantRabMAb

Recombinant FITC Anti-GAPDH antibody [EPR16891] (ab224005)

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Immunocytochemistry/ Immunofluorescence - FITC Anti-GAPDH antibody [EPR16891] (ab224005)
  • FITC Anti-GAPDH antibody [EPR16891] (ab224005)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • FITC Rabbit monoclonal [EPR16891] to GAPDH
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm

Overview

  • Product name

    FITC Anti-GAPDH antibody [EPR16891]
    See all GAPDH primary antibodies

  • Description

    FITC Rabbit monoclonal [EPR16891] to GAPDH

  • Host species

    Rabbit

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Fish, Monkey, Xenopus tropicalis

  • Immunogen

    Recombinant fragment within Mouse GAPDH aa 100 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P16858

  • Positive control

    • ICC/IF: HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab224005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)
Notes
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

Target

  • Function

    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

  • Sequence similarities

    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

  • Post-translational
    modifications

    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.

  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
    • aging associated gene 9 protein antibody
    • Aging-associated gene 9 protein antibody
    • BARS-38 antibody
    • cb609 antibody
    • EC 1.2.1.12 antibody
    • Epididymis secretory sperm binding protein Li 162eP antibody
    • G3P_HUMAN antibody
    • G3PD antibody
    • G3PDH antibody
    • GAPD antibody
    • GAPDH antibody
    • Glyceraldehyde 3 phosphate dehydrogenase antibody
    • glyceraldehyde 3-PDH antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • HEL-S-162eP antibody
    • KNC-NDS6 antibody
    • MGC102544 antibody
    • MGC102546 antibody
    • MGC103190 antibody
    • MGC103191 antibody
    • MGC105239 antibody
    • MGC127711 antibody
    • MGC88685 antibody
    • OCAS, p38 component antibody
    • OCT1 coactivator in S phase, 38-KD component antibody
    • peptidyl cysteine S nitrosylase GAPDH antibody
    • Peptidyl-cysteine S-nitrosylase GAPDH antibody
    • wu:fb33a10 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - FITC Anti-GAPDH antibody [EPR16891] (ab224005)
    Immunocytochemistry/ Immunofluorescence - FITC Anti-GAPDH antibody [EPR16891] (ab224005)

    ab224005 staining GAPDH in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab224005 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • FITC Anti-GAPDH antibody [EPR16891] (ab224005)
    FITC Anti-GAPDH antibody [EPR16891] (ab224005)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab224005? Please let us know so that we can cite the reference in this datasheet.

ab224005 has not yet been referenced specifically in any publications.

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