FITC Anti-GAPDS antibody [Hs-8] (ab240823)
![Flow Cytometry - FITC Anti-GAPDS antibody [Hs-8] (ab240823)](https://www.abcam.com/ps/products/240/ab240823/Images/ab240823-319518-ab240823.jpg "Flow Cytometry - FITC Anti-GAPDS antibody [Hs-8] (ab240823)")
Key features and details
- FITC Mouse monoclonal [Hs-8] to GAPDS
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgM
Overview
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Product name
FITC Anti-GAPDS antibody [Hs-8]
See all GAPDS primary antibodies -
Description
FITC Mouse monoclonal [Hs-8] to GAPDS -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human GAPDS. (Freshly ejaculated human sperms washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and used for immunization).
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Positive control
- Flow Cyt: Acrosomes of human sperms.
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General notes
This product was previously labelled as GAPDHS
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 8.0
Preservative: 0.0975% Sodium azide
Constituent: Tris buffered saline -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. -
Clonality
Monoclonal -
Clone number
Hs-8 -
Isotype
IgM -
Research areas
Associated products
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab240823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. Analysis of sperm cells using 20 µl reagent / 100 µl cell suspension. |
Target
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Function
May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility. -
Tissue specificity
Testis specific. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
Domain
The testis-specific N-terminal extension mediates tight association with the cytoskeletal fibrous sheath of the spermatozoa flagellum, possibly via interchain disulfide-bonding of Cys-21 with sheath components. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 26330 Human
- Omim: 609169 Human
- SwissProt: O14556 Human
- Unigene: 248017 Human
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Alternative names
- G3PT_HUMAN antibody
- GAPD2 antibody
- GAPDH 2 antibody
see all
Images
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Flow Cytometry - FITC Anti-GAPDS antibody [Hs-8] (ab240823)
Flow cytometric analysis of acrosomes of human sperms labeling GAPDS with ab240823.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab240823 has not yet been referenced specifically in any publications.
