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  1. Link

    fitc-glycophorin-a-antibody-ab112201.pdf

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FITC Anti-Glycophorin A antibody (ab112201)

  • Datasheet
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Flow Cytometry - FITC Anti-Glycophorin A antibody (ab112201)

    Key features and details

    • FITC Rabbit polyclonal to Glycophorin A
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG

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    Recombinant Human Glycophorin A protein (ab114330)
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    Anti-PLA2R antibody (ab194256)

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    Overview

    • Product name

      FITC Anti-Glycophorin A antibody
      See all Glycophorin A primary antibodies
    • Description

      FITC Rabbit polyclonal to Glycophorin A
    • Host species

      Rabbit
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Full length protein corresponding to Human Glycophorin A.

    • Positive control

      • Human red blood cells
    • General notes


      The 23 residue hydrophobic transmembrane domain has been shown to mediate non-covalent dimerization of the protein under conditions of SDS page (and in other detergents).

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituent: 99% PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      ab112201 was purified from antisera via Protein G Chromatography.
    • Primary antibody notes

      The 23 residue hydrophobic transmembrane domain has been shown to mediate non-covalent dimerization of the protein under conditions of SDS page (and in other detergents).
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Blood
      • Blood Cell Antigens
      • RBC Antigens
      • Cardiovascular
      • Blood
      • Blood Cell Antigens
      • RBC Antigens
      • Blood Group Antigens

    Associated products

    • Isotype control

      • FITC-Rabbit IgG - Isotype Control (ab37406)
    • Recombinant Protein

      • Recombinant Human Glycophorin A protein (ab114330)

    Applications

    Our Abpromise guarantee covers the use of ab112201 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 0.2µg for 106 cells.

    ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
    • Sequence similarities

      Belongs to the glycophorin A family.
    • Post-translational
      modifications

      The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
    • Cellular localization

      Cell membrane. Appears to be colocalized with SLC4A1.
    • Target information above from: UniProt accession P02724 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2993 Human
      • Omim: 111300 Human
      • SwissProt: P02724 Human
      • Unigene: 434973 Human
      • Alternative names

        • AI853584 antibody
        • Blood group--MN locus antibody
        • CD_antigen=CD235a antibody
        • CD235a antibody
        • GLPA_HUMAN antibody
        • Glycophorin A (MNS blood group) antibody
        • Glycophorin A antibody
        • Glycophorin A, included antibody
        • Glycophorin-A antibody
        • GlycophorinA antibody
        • GPA antibody
        • GPErik antibody
        • GpMiIII antibody
        • GPSAT antibody
        • GYPA antibody
        • GYPA, included antibody
        • HGpMiIII antibody
        • HGpMiV antibody
        • HGpMiX antibody
        • HGpMiXI antibody
        • HGpSta(C) antibody
        • MN antibody
        • MN sialoglycoprotein antibody
        • MNS antibody
        • PAS-2 antibody
        • PAS2 antibody
        • Sialoglycoprotein alpha antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-Glycophorin A antibody (ab112201)
        Flow Cytometry - FITC Anti-Glycophorin A antibody (ab112201)
        ab112201, at 0.2 µg/106 cells, staining Glycophorin A in Human red blood cells by Flow Cytometry.
        Isotypic Control: FITC Rabbit IgG
        Percentage of cells stained above control: 99.2 %.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab112201? Please let us know so that we can cite the reference in this datasheet.

      ab112201 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      I would like to enquire a question. Are there any antibody of Anti-Glycophorin A antibody [HIR2] (ab15009) conjugated with FITC and antibody of Anti-Glycophorin A antibody [HIR2] (ab15009) conjugated with Phycoerythrin?
      If the antibody I mentioned is not available, is it possible to obtain those antibodies I mentioned, meaning specifically made it?
      If there is the antibody I mentioned or it is possible to specifically made the antibodies I mentioned, I would like to enquire a few questions as listed below:-
      (a) What is the concentration of the antibody mentioned?
      (b) What would the price be?
      (c) When will it be able to arrive if I were to order it by this week (Address: London, United Kingdom)?
      In addition, I would like to know the concentration for these antibodies as well.
      (a) Anti-Glycophorin A antibody [HIR2] (FITC) - Aminoterminal end (ab52452)
      (b) Anti-Glycophorin A + B antibody [HIR2] (Phycoerythrin) (ab26016)
      Thank you for taking up your time.
      Hope to hear from you soon.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 12 2012

      Answer

      Thank you for contacting us.

      We have 4, FITC conjugated anti Glycophorin A antibodies.

      https://www.abcam.com/Glycophorin-A-antibody-YTH89-1-FITC-ab28082.html
      https://www.abcam.com/Glycophorin-A-antibody-FITC-ab112201.html
      https://www.abcam.com/Glycophorin-A-antibody-HIR2-FITC-Aminoterminal-end-ab52452.html
      https://www.abcam.com/Glycophorin-A-antibody-CLB-AME-1-FITC-ab51531.html

      3 Phycoerythrin conjugated abs

      https://www.abcam.com/Glycophorin-A-antibody-CLB-AME-1-Phycoerythrin-ab51632.html
      https://www.abcam.com/Glycophorin-A-antibody-YTH89-1-Phycoerythrin-ab33387.html
      https://www.abcam.com/Glycophorin-A-B-antibody-HIR2-Phycoerythrin-ab26016.html

      The price for these antibodies for 1 vial would be

      ab28082; £283 per 100 microgram
      ab51632: £227 per 500 micro litre
      ab112201; £227 per 100 microgram
      ab33387: £227 50 tests
      ab52452: £227 per 100 tests
      ab51531; £227 per 100 tests
      ab26016: £227 per 100 tests

      ab26016 the concentration is 0.01mg/ml

      All other information is present on the datasheets. The product will be dispatched same day for next day delivery.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Jun 12 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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