FITC Anti-H2-Ea antibody [M5/114.15.2] (ab93561)
Key features and details
- FITC Rat monoclonal [M5/114.15.2] to H2-Ea
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Overview
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Product name
FITC Anti-H2-Ea antibody [M5/114.15.2]
See all H2-Ea primary antibodies -
Description
FITC Rat monoclonal [M5/114.15.2] to H2-Ea -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab93561 reacts with the mouse MHC Class II, both I-A and I-E subregion-encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u but not from mice carrying the H-2s or H-2f haplotypes. -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse H2-Ea.
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Positive control
- Mouse C57BI/6 splenocytes
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General notes
The M5/114.15.2 antibody is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes.
This product was previously labelled as MHC Class II IA + IE
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.16% Sodium phosphate, 0.1% Gelatin -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
The M5/114.15.2 antibody is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes. -
Clonality
Monoclonal -
Clone number
M5/114.15.2 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Target
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Relevance
The MHC Class II IA + IE molcules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 b, d, q, r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. -
Cellular localization
Single-pass type I membrane protein -
Database links
- Entrez Gene: 111861 Mouse
- SwissProt: P01904 Mouse
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Alternative names
- AI323765 antibody
- E alpha f antibody
- H 2Ea antibody
see all
Datasheets and documents
References (9)
ab93561 has been referenced in 9 publications.
- Zhang S et al. Salmonella infection leads to severe intestinal inflammation and increased CD4+FoxP3+ Treg cells in streptozotocin-induced hyperglycemic mice. Mol Med Rep 19:5377-5385 (2019). PubMed: 31059096
- Meng Y et al. Paeonol ameliorates imiquimod-induced psoriasis-like skin lesions in BALB/c mice by inhibiting the maturation and activation of dendritic cells. Int J Mol Med 39:1101-1110 (2017). PubMed: 28339016
- Zeng SL et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). PubMed: 25936350
- Unternaehrer JJ et al. The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A 104:234-9 (2007). PubMed: 17190803
- Gueirard P et al. Bordetella bronchiseptica persists in the nasal cavities of mice and triggers early delivery of dendritic cells in the lymph nodes draining the lower and upper respiratory tract. Infect Immun 71:4137-43 (2003). PubMed: 12819105
- Bagavant H et al. Induction and immunohistology of autoimmune ovarian disease in cynomolgus macaques (Macaca fascicularis). Am J Pathol 160:141-9 (2002). PubMed: 11786408
- Li C et al. Cooperative interaction of Ig(alpha) and Ig(beta) of the BCR regulates the kinetics and specificity of antigen targeting. Int Immunol 14:1179-91 (2002). IP . PubMed: 12356683
- Mendiratta SK et al. Analysis of T-cell hybridomas with an unusual MHC class II-dependent ligand specificity. Immunology 89:238-44 (1996). PubMed: 8943720
- Germain RN et al. A single monoclonal anti-Ia antibody inhibits antigen-specific T cell proliferation controlled by distinct Ir genes mapping in different H-2 I subregions. J Immunol 128:1409-13 (1982). PubMed: 6173436
