Key features and details
- FITC Rabbit polyclonal to HA tag
- Suitable for: WB, ICC/IF
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Product nameFITC Anti-HA tag antibody
See all HA tag primary antibodies
DescriptionFITC Rabbit polyclonal to HA tag
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Species independent
YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH.
Molar F/P ratio is 3.7:1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
Our Abpromise guarantee covers the use of ab1208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
ab1208 staining Ha tag in human breast cancer cells (HA tagged expression plasmid) by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.2% Triton X-100 in PBS and then blocked using 1% BSA and 3% FBS in PBS for 30 minutes at room temperature. Samples were then incubated with primary antibody at 1/50 for 1 hour.
ab1208 has been referenced in 3 publications.
- Chung SH et al. Factors Impacting Efficacy of AAV-Mediated CRISPR-Based Genome Editing for Treatment of Choroidal Neovascularization. Mol Ther Methods Clin Dev 17:409-417 (2020). PubMed: 32128346
- Polpitiya Arachchige S et al. Analysis of Select Herpes Simplex Virus 1 (HSV-1) Proteins for Restriction of Human Immunodeficiency Virus Type 1 (HIV-1): HSV-1 gM Protein Potently Restricts HIV-1 by Preventing Intracellular Transport and Processing of Env gp160. J Virol 92:N/A (2018). PubMed: 29093081
- Decker CE et al. Tmem178 acts in a novel negative feedback loop targeting NFATc1 to regulate bone mass. Proc Natl Acad Sci U S A 112:15654-9 (2015). PubMed: 26644563