FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (ab69545)

Reviews (1)Q&A (1)References (4)
Flow Cytometry - FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (ab69545)

    Key features and details

    • FITC Mouse monoclonal [HO-1-2] to Heme Oxygenase 1
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG2b

    Overview

    • Product name

      FITC Anti-Heme Oxygenase 1 antibody [HO-1-2]
      See all Heme Oxygenase 1 primary antibodies

    • Description

      FITC Mouse monoclonal [HO-1-2] to Heme Oxygenase 1

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Full length native protein (purified) (Rat)

    • Positive control

      • Jurkat cells

    • General notes

      This product was changed from ascites to tissue culture supernatant on 14th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab69545 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use a concentration of 100 µg/ml.

    ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

     
    Notes
    Flow Cyt
    Use a concentration of 100 µg/ml.

    ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.

    • Sequence similarities

      Belongs to the heme oxygenase family.

    • Cellular localization

      Microsome. Endoplasmic reticulum.
    • Information by UniProt

    • Database links

      • Alternative names

        • 32 kD antibody
        • bK286B10 antibody
        • D8Wsu38e antibody
        • heat shock protein 32 kD antibody
        • heat shock protein 32kD antibody
        • Heat shock protein antibody
        • Heme oxygenase (decycling) 1 antibody
        • Heme oxygenase 1 antibody
        • Hemox antibody
        • HMOX 1 antibody
        • Hmox antibody
        • Hmox1 antibody
        • HMOX1_HUMAN antibody
        • HO 1 antibody
        • HO antibody
        • HO-1 antibody
        • HO1 antibody
        • Hsp32 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (ab69545)
        Flow Cytometry - FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (ab69545)
        Flow Cytometry of 106 Jurkat cells stained using ab69545 at a concentration of 100 µg/mL.

      References (4)

      Publishing research using ab69545? Please let us know so that we can cite the reference in this datasheet.

      ab69545 has been referenced in 4 publications.

      • Lei L  et al. Dihydroquercetin Activates AMPK/Nrf2/HO-1 Signaling in Macrophages and Attenuates Inflammation in LPS-Induced Endotoxemic Mice. Front Pharmacol 11:662 (2020). PubMed: 32508636
      • Chiang N  et al. Inhaled carbon monoxide accelerates resolution of inflammation via unique proresolving mediator-heme oxygenase-1 circuits. J Immunol 190:6378-88 (2013). PubMed: 23650615
      • Walther M  et al. HMOX1 gene promoter alleles and high HO-1 levels are associated with severe malaria in Gambian children. PLoS Pathog 8:e1002579 (2012). Flow Cyt ; Human . PubMed: 22438807
      • Mougiakakos D  et al. The impact of inflammatory licensing on heme oxygenase-1-mediated induction of regulatory T cells by human mesenchymal stem cells. Blood 117:4826-35 (2011). Flow Cyt ; Human . PubMed: 21389316

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Heme Oxygenase 1 antibody [HO-1-2] (FITC)

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (hepatocytes)
      Permeabilization
      Yes - 0.1% Triton X-100
      Specification
      hepatocytes
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 22 2015

      Question

      HIgh background in Flow cytometry
      No isotype control
      ab used at 5ug/ml

      Read More
      Answer

      Thank you very much for contacting us.

      As discussed please attach the protocol and send it to us, as per your earlier conveniance.

      Many thanks!

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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