FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (ab69545)
![Flow Cytometry - FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (ab69545)](https://www.abcam.com/ps/products/69/ab69545/Images/ab69545-83902-anti-heme-oxygenase-1-antibody-ho-1-2-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (ab69545)")
Key features and details
- FITC Mouse monoclonal [HO-1-2] to Heme Oxygenase 1
- Suitable for: Flow Cyt
- Reacts with: Mouse, Rat, Guinea pig, Hamster, Dog, Human, Monkey
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Overview
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Product name
FITC Anti-Heme Oxygenase 1 antibody [HO-1-2]
See all Heme Oxygenase 1 primary antibodies -
Description
FITC Mouse monoclonal [HO-1-2] to Heme Oxygenase 1 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Guinea pig, Hamster, Dog, Human, Monkey -
Immunogen
Full length native protein (purified) (Rat)
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Positive control
- Jurkat cells
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General notes
This product was changed from ascites to tissue culture supernatant on 14th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
HO-1-2 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab69545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use a concentration of 100 µg/ml. ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Target
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Function
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. -
Sequence similarities
Belongs to the heme oxygenase family. -
Cellular localization
Microsome. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 442987 Dog
- Entrez Gene: 101833770 Hamster
- Entrez Gene: 3162 Human
- Entrez Gene: 15368 Mouse
- Entrez Gene: 24451 Rat
- Omim: 141250 Human
- SwissProt: P09601 Human
- SwissProt: P14901 Mouse
see all -
Alternative names
- 32 kD antibody
- bK286B10 antibody
- D8Wsu38e antibody
see all
Images
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Flow Cytometry - FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (ab69545)Flow Cytometry of 106 Jurkat cells stained using ab69545 at a concentration of 100 µg/mL.
Datasheets and documents
References (3)
ab69545 has been referenced in 3 publications.
- Chiang N et al. Inhaled carbon monoxide accelerates resolution of inflammation via unique proresolving mediator-heme oxygenase-1 circuits. J Immunol 190:6378-88 (2013). PubMed: 23650615
- Walther M et al. HMOX1 gene promoter alleles and high HO-1 levels are associated with severe malaria in Gambian children. PLoS Pathog 8:e1002579 (2012). Flow Cyt ; Human . PubMed: 22438807
- Mougiakakos D et al. The impact of inflammatory licensing on heme oxygenase-1-mediated induction of regulatory T cells by human mesenchymal stem cells. Blood 117:4826-35 (2011). Flow Cyt ; Human . PubMed: 21389316
