For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    fitc-heme-oxygenase-1-antibody-ho-1-2-ab69545.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Other
Share by email

FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (ab69545)

  • Datasheet
Reviews (1)Q&A (1)References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Flow Cytometry - FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (ab69545)

    Key features and details

    • FITC Mouse monoclonal [HO-1-2] to Heme Oxygenase 1
    • Suitable for: Flow Cyt
    • Reacts with: Mouse, Rat, Guinea pig, Hamster, Dog, Human, Monkey
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG2b

    You may also be interested in

    Protein
    Product image
    Recombinant Human Heme Oxygenase 1 protein (ab85243)

    View more associated products

    Overview

    • Product name

      FITC Anti-Heme Oxygenase 1 antibody [HO-1-2]
      See all Heme Oxygenase 1 primary antibodies
    • Description

      FITC Mouse monoclonal [HO-1-2] to Heme Oxygenase 1
    • Host species

      Mouse
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Guinea pig, Hamster, Dog, Human, Monkey
    • Immunogen

      Full length native protein (purified) (Rat)

    • Positive control

      • Jurkat cells
    • General notes

      This product was changed from ascites to tissue culture supernatant on 14th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      Purified from TCS
    • Clonality

      Monoclonal
    • Clone number

      HO-1-2
    • Isotype

      IgG2b
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Other
      • Cardiovascular
      • Blood
      • Platelets
      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • NFkB Pathway
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • NFkB pathway
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Inflammatory mediators
      • Cardiovascular
      • Vasculature
      • Endothelium
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Neurodegenerative disease
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Types of disease
      • Heart disease

    Associated products

    • Alternative Versions

      • PE Anti-Heme Oxygenase 1 antibody [HO-1-2] (ab83214)
    • Isotype control

      • FITC Mouse IgG2b [PLPV219] - Isotype Control (ab91368)
    • Recombinant Protein

      • Recombinant Human Heme Oxygenase 1 protein (ab85243)

    Applications

    Our Abpromise guarantee covers the use of ab69545 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use a concentration of 100 µg/ml.

    ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
    • Sequence similarities

      Belongs to the heme oxygenase family.
    • Cellular localization

      Microsome. Endoplasmic reticulum.
    • Target information above from: UniProt accession P09601 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 442987 Dog
      • Entrez Gene: 101833770 Hamster
      • Entrez Gene: 3162 Human
      • Entrez Gene: 15368 Mouse
      • Entrez Gene: 24451 Rat
      • Omim: 141250 Human
      • SwissProt: P09601 Human
      • SwissProt: P14901 Mouse
      • SwissProt: P06762 Rat
      • Unigene: 517581 Human
      • Unigene: 276389 Mouse
      • Unigene: 3160 Rat
      see all
    • Alternative names

      • 32 kD antibody
      • bK286B10 antibody
      • D8Wsu38e antibody
      • heat shock protein 32 kD antibody
      • heat shock protein 32kD antibody
      • Heat shock protein antibody
      • Heme oxygenase (decycling) 1 antibody
      • Heme oxygenase 1 antibody
      • Hemox antibody
      • HMOX 1 antibody
      • Hmox antibody
      • Hmox1 antibody
      • HMOX1_HUMAN antibody
      • HO 1 antibody
      • HO antibody
      • HO-1 antibody
      • HO1 antibody
      • Hsp32 antibody
      see all

    Images

    • Flow Cytometry - FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (ab69545)
      Flow Cytometry - FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (ab69545)
      Flow Cytometry of 106 Jurkat cells stained using ab69545 at a concentration of 100 µg/mL.

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab69545? Please let us know so that we can cite the reference in this datasheet.

    ab69545 has been referenced in 3 publications.

    • Chiang N  et al. Inhaled carbon monoxide accelerates resolution of inflammation via unique proresolving mediator-heme oxygenase-1 circuits. J Immunol 190:6378-88 (2013). PubMed: 23650615
    • Walther M  et al. HMOX1 gene promoter alleles and high HO-1 levels are associated with severe malaria in Gambian children. PLoS Pathog 8:e1002579 (2012). Flow Cyt ; Human . PubMed: 22438807
    • Mougiakakos D  et al. The impact of inflammatory licensing on heme oxygenase-1-mediated induction of regulatory T cells by human mesenchymal stem cells. Blood 117:4826-35 (2011). Flow Cyt ; Human . PubMed: 21389316

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Heme Oxygenase 1 antibody [HO-1-2] (FITC)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (hepatocytes)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    hepatocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 22 2015

    Question

    HIgh background in Flow cytometry
    No isotype control
    ab used at 5ug/ml

    Read More

    Abcam community

    Verified customer

    Asked on Feb 29 2012

    Answer

    Thank you very much for contacting us.

    As discussed please attach the protocol and send it to us, as per your earlier conveniance.

    Many thanks!

    Read More

    Abcam Scientific Support

    Answered on Feb 29 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.