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    fitc-hemoglobin-antibody-ab19361.pdf

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Cardiovascular Blood Blood Cell Antigens RBC Antigens
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FITC Anti-Hemoglobin antibody (ab19361)

  • Datasheet
  • SDS
Submit a review Q&A (3)

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • FITC Goat polyclonal to Hemoglobin
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

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Overview

  • Product name

    FITC Anti-Hemoglobin antibody
    See all Hemoglobin primary antibodies
  • Description

    FITC Goat polyclonal to Hemoglobin
  • Host species

    Goat
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Human Hemoglobin

  • General notes

    F/P ratio is 3 to 7.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Cardiovascular
    • Blood
    • Other
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Blood cell antigens ELISA kits

Associated products

  • Isotype control

    • FITC Goat IgG - Isotype control (ab37374)

Target

  • Function

    Involved in oxygen transport from the lung to the various peripheral tissues.
  • Tissue specificity

    Red blood cells.
  • Involvement in disease

    Defects in HBA1/HBA2 may be a cause of Heinz body anemias (HEIBAN) [MIM:140700]. This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency.
    Defects in HBA1/HBA2 are the cause of alpha-thalassemia (A-THAL) [MIM:604131]. The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non-deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers.
    Note=Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
  • Sequence similarities

    Belongs to the globin family.
  • Post-translational
    modifications

    The initiator Met is not cleaved in variant Thionville and is acetylated.
  • Target information above from: UniProt accession P69905 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3039 Human
    • Entrez Gene: 3040 Human
    • Entrez Gene: 3043 Human
    • Entrez Gene: 3047 Human
    • Entrez Gene: 693930 Rhesus monkey
    • Omim: 141800 Human
    • Omim: 141900 Human
    • Omim: 142200 Human
    • SwissProt: P69907 Chimpanzee
    • SwissProt: P01923 Gorilla
    • SwissProt: P68871 Human
    • SwissProt: P69891 Human
    • SwissProt: P69905 Human
    • SwissProt: P63108 Rhesus monkey
    • Unigene: 449630 Human
    • Unigene: 523443 Human
    • Unigene: 654744 Human
    see all
  • Alternative names

    • 3-prime alpha-globin gene antibody
    • A gamma globin antibody
    • Alpha 1 globin antibody
    • Alpha 2 globin antibody
    • Alpha 2 globin chain antibody
    • Alpha globin antibody
    • alpha one globin antibody
    • alpha-1 globin antibody
    • Alpha-globin antibody
    • Beta globin antibody
    • CD113t C antibody
    • CD31 antibody
    • Delta globin antibody
    • Erythremia, beta-globin type, included antibody
    • Gamma 1 globin antibody
    • Gamma A hemoglobin antibody
    • Gamma globin antibody
    • Hb F Agamma antibody
    • HBA 1 antibody
    • HBA 2 antibody
    • HBA antibody
    • HBA T3 antibody
    • HBA-T2 antibody
    • HBA_HUMAN antibody
    • HBA1 antibody
    • HBA2 antibody
    • HBB antibody
    • Hbb-y antibody
    • HBD antibody
    • Hbe1 antibody
    • HBG 1 antibody
    • HBG antibody
    • HBG1 antibody
    • HBGA antibody
    • HBGR antibody
    • HBH antibody
    • Hemoglobin alpha 1 antibody
    • Hemoglobin alpha 1 chain antibody
    • hemoglobin alpha 1 globin chain antibody
    • hemoglobin alpha 2 antibody
    • Hemoglobin alpha chain antibody
    • Hemoglobin alpha locus antibody
    • Hemoglobin alpha locus 1 antibody
    • hemoglobin alpha-1 chain antibody
    • Hemoglobin beta antibody
    • Hemoglobin beta chain antibody
    • Hemoglobin beta chain complex antibody
    • Hemoglobin beta locus antibody
    • Hemoglobin gamma 1 chain antibody
    • Hemoglobin gamma A antibody
    • Hemoglobin gamma A chain antibody
    • Hemoglobin gamma antibody
    • Hemoglobin subunit alpha antibody
    • Hemoglobin subunit beta antibody
    • Hemoglobin subunit gamma 1 antibody
    • hemoglobin, gamma, regulator of antibody
    • Hemoglobin--gamma locus, 136 alanaine antibody
    • HSGGL1 antibody
    • LVV-hemorphin-7 antibody
    • Methemoglobinemia, beta-globin type, included antibody
    • MGC126895 antibody
    • MGC126897 antibody
    • Minor alpha-globin locus antibody
    • PRO2979 antibody
    see all

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab19361? Please let us know so that we can cite the reference in this datasheet.

    ab19361 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Does it react with all haemoglobin chains or with rather one of the alpha, beta, gamma chains?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 14 2013

    Answer

    Thank you for contacting us.

    ab19361 Anti-Hemoglobin antibody (FITC) should react with all hemoglobin chains.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 14 2013

    Question

    Signal in negative control cells (HSCs) in flow cytometry.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 27 2012

    Answer

    Thank you very much for your call today and for letting us know about the trouble with ab19361.

    Please keep me updated about the results after further diluting the antibody, and if you'd like to try an isotype control we have the goat IgG FITC conjugate Ab37374-

    https://www.abcam.com/Goat-IgG-FITC-Isotype-control-ab37374.html

    This will also help determine what part of the signal is coming from non-specific binding between the IgG and the cells.

    I look forward to hearing from you. If you have further questions or need anything else from us, please let me know and I'll be happy to help.

    Read More

    Abcam Scientific Support

    Answered on Sep 27 2012

    Question

    Our lab has asked for Hemoglobin antibodies we can test to see if they will react with mouse hemoglobin. We still need your expertise. We need an antibody that detects adult mouse hemoglobin and we can use it in FACS . There are 3 antibodies we might be interested in: the PBH1 (ab9249) the advantage here is the fact that this Ab was tested in FACS the disadvantage is that since this is a mouse monoclonal the secondary antibodies will be against mouse and that will not work on mouse cells, isn't? The other 2 are: the Hb Ab FITC( ab19191) or the Hb Ab FITC (ab19361) which are polyclonal sheepor goat Ab the advantage is that they are directly conjugated Ab to FITC the disadvantage is that thae were not tested for FACS and are polyclonal sheep or goat. What is your advice, apart from this 3 Ab I choose, do you think there are other better options for hemoglobin antibodies to be tested in primary mouse cells? Initially we have asked about the HBF1 (ab20517) but this is a fetal Hb Ab and we need to detect the adult Hb. And the last question: your offer was a 20% discount for testing purposes, and that no matter which antibody we buy? Thank you very much for your help

    Read More

    Abcam community

    Verified customer

    Asked on Jun 02 2006

    Answer

    Thank you for getting back to me. I think that your best options are the antibodies that you have highlighted. The application of ab9249 Hemoglobin antibody [PBH1] to your murine cells is likely to cause you some difficulties given the potential reactivity of the secondary with endogenous mouse IgG. Furthermore as you mention ab19191 and ab19361 whilst they are directly fluorescently conjugated they are yet to be tested by FACS. At Abcam we are always interested in seeing our antibodies tested in new applications against new species. Given the potential artificial reactivity of ab9249 I consider your best option ab19191 and ab19361. I am prepared to offer you a 20% discount against the purchase of either these antibodies. Should you wish to take me up on this offer and prepared to provide me with feedback of how the antibody performs please email me and I will provide you with a unique discount code. Abreviews can be left by navigating to the antibody datasheet and clicking on the "Abreview" tab. In return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image).

    Read More

    Abcam Scientific Support

    Answered on Jun 05 2006

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